primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	psbI-trnS-GCU	1	GTAATCCTGGGCGTGAAGAA	CGAATTATCCGTACCGAGGG	20	20	50.0	55.0	57.6	57.7	0.0	0.0	0.739499	9951	10195	308-632	0.666667	72.8
psbI-trnS-GCU_p2	psbI-trnS-GCU	2	GTAATCCTGGGCGTGAAGAA	GATGGCTGAGTGGACGAAAG	20	20	50.0	55.0	57.6	58.6	0.0	0.0	1.032846	9951	10195	351-675	0.666667	70.4
psbI-trnS-GCU_p3	psbI-trnS-GCU	3	GTAATCCTGGGCGTGAAGAA	TTCGAATCCCTCTCTTTCCG	20	20	50.0	50.0	57.6	57.0	0.0	0.0	1.358116	9951	10195	288-612	0.666667	67.8
psbI-trnS-GCU_p4	psbI-trnS-GCU	4	GTAATCCTGGGCGTGAAGAA	TTATCCGTACCGAGGGTTCG	20	20	50.0	55.0	57.6	59.0	0.0	6.8	1.372318	9951	10195	304-628	0.666667	67.7
psbI-trnS-GCU_p5	psbI-trnS-GCU	5	GTAATCCTGGGCGTGAAGAA	CGAAAGCGTCGGATTGCTAA	20	20	50.0	50.0	57.6	59.0	0.0	7.9	1.400128	9951	10195	337-661	0.666667	67.5
trnS-GCU-trnG-GCC_p1	trnS-GCU-trnG-GCC	1	AATTCCAAGTCATTGCCGGA	ACGAATCACACTTTTACCACT	20	21	45.0	38.1	57.8	55.5	5.0	0.0	3.750219	10284	12238	1703-2099	0.666667	48.7
trnS-GCU-trnG-GCC_p2	trnS-GCU-trnG-GCC	2	ATTCCAAGTCATTGCCGGAA	ACGAATCACACTTTTACCACT	20	21	45.0	38.1	57.8	55.5	5.0	0.0	3.750219	10284	12238	1702-2098	0.666667	48.7
trnS-GCU-trnG-GCC_p3	trnS-GCU-trnG-GCC	3	AATTCCAAGTCATTGCCGGA	GAACGAATCACACTTTTACCACT	20	23	45.0	39.1	57.8	57.3	5.0	0.0	3.882934	10284	12238	1705-2101	0.666667	47.6
trnS-GCU-trnG-GCC_p4	trnS-GCU-trnG-GCC	4	ATTCCAAGTCATTGCCGGAA	AGAACGAATCACACTTTTACCAC	20	23	45.0	39.1	57.8	57.3	5.0	0.0	3.882934	10284	12238	1705-2101	0.666667	47.6
trnS-GCU-trnG-GCC_p5	trnS-GCU-trnG-GCC	5	ATTCCAAGTCATTGCCGGAA	GAACGAATCACACTTTTACCACT	20	23	45.0	39.1	57.8	57.3	5.0	0.0	3.882934	10284	12238	1704-2100	0.666667	47.6
trnG-GCC-trnR-UCU_p1	trnG-GCC-trnR-UCU	1	AGCCTTCCAAGCTAACGATG	GCGAGTTGCTCAAACAATCC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.060735	13011	13706	1378-2735	1.000000	89.5
trnG-GCC-trnR-UCU_p2	trnG-GCC-trnR-UCU	2	CCTAGCCTTCCAAGCTAACG	GCGAGTTGCTCAAACAATCC	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.089572	13011	13706	1381-2738	1.000000	89.3
trnG-GCC-trnR-UCU_p3	trnG-GCC-trnR-UCU	3	TTTCGCTCACGTCTTCTAGC	GCGAGTTGCTCAAACAATCC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.052355	13011	13706	1506-2863	1.000000	89.1
trnG-GCC-trnR-UCU_p4	trnG-GCC-trnR-UCU	4	AAGGGATCATCGGCTCCATA	GCGAGTTGCTCAAACAATCC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.060924	13011	13706	2048-2048	0.333333	52.8
trnG-GCC-trnR-UCU_p5	trnG-GCC-trnR-UCU	5	TAAGGGATCATCGGCTCCAT	GCGAGTTGCTCAAACAATCC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.060924	13011	13706	2049-2049	0.333333	52.8
atpF-atpH_p1	atpF-atpH	1	CAGTGACCCAAGGAAACGAA	CCGTTATTGCTGCTGGTTTG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.056953	16949	18634	887-2053	1.000000	91.5
atpF-atpH_p2	atpF-atpH	2	CAGTGACCCAAGGAAACGAA	CGTTATTGCTGCTGGTTTGG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.056953	16949	18634	886-2052	1.000000	91.5
atpF-atpH_p3	atpF-atpH	3	CAGTGACCCAAGGAAACGAA	ATTTCTGCCGCTTCCGTTAT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.076593	16949	18634	900-2066	1.000000	91.4
atpF-atpH_p4	atpF-atpH	4	CAGTGACCCAAGGAAACGAA	AGGCCAAGCTGTAGAAGGTA	20	20	50.0	50.0	58.0	58.0	0.0	5.0	0.085585	16949	18634	817-1983	1.000000	91.3
atpF-atpH_p5	atpF-atpH	5	CAGTGACCCAAGGAAACGAA	ATCCATTGATTTCTGCCGCT	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.101177	16949	18634	908-2074	1.000000	91.2
trnE-UUC-trnT-GGU_p1	trnE-UUC-trnT-GGU	1	GCTGCCTCCTTGAAAGAGAG	GAACCGATGACTTACGCCTT	20	20	55.0	50.0	58.3	58.0	0.0	0.0	0.269359	36715	38523	1912-1912	0.333333	51.2
trnE-UUC-trnT-GGU_p2	trnE-UUC-trnT-GGU	2	TGTCCTGAACCACTAGACGA	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	57.7	58.0	0.0	0.0	0.287509	36715	38523	1891-1891	0.333333	51.0
trnE-UUC-trnT-GGU_p3	trnE-UUC-trnT-GGU	3	GCTGCCTCCTTGAAAGAGAG	CCATGGCGTTACTCTACCAC	20	20	55.0	55.0	58.3	58.1	0.0	0.0	0.327456	36715	38523	1891-1891	0.333333	50.7
trnE-UUC-trnT-GGU_p4	trnE-UUC-trnT-GGU	4	TGTCCTGAACCACTAGACGA	CCATGGCGTTACTCTACCAC	20	20	50.0	55.0	57.7	58.1	0.0	0.0	0.345606	36715	38523	1870-1870	0.333333	50.6
trnE-UUC-trnT-GGU_p5	trnE-UUC-trnT-GGU	5	GCTGCCTCCTTGAAAGAGAG	TGGCGTTACTCTACCACTGA	20	20	55.0	50.0	58.3	58.1	0.0	0.0	0.349611	36715	38523	1888-1888	0.333333	50.5
psbZ-trnG-UCC_p1	psbZ-trnG-UCC	1	TCCTTAATTCTCTCATCTCTTGA	CGTCTTCTCCTTGGCAAAGA	23	20	34.8	50.0	54.3	57.8	0.0	1.1	6.894900	43988	45503	1247-2086	1.000000	52.0
psbZ-trnG-UCC_p2	psbZ-trnG-UCC	2	GGTATCCTTAATTCTCTCATCTCT	CGTCTTCTCCTTGGCAAAGA	24	20	37.5	50.0	55.0	57.8	0.0	1.1	7.178777	43988	45503	1251-2090	1.000000	52.0
psbZ-trnG-UCC_p3	psbZ-trnG-UCC	3	GGTATCCTTAATTCTCTCATCTCTTG	CGTCTTCTCCTTGGCAAAGA	26	20	38.5	50.0	56.9	57.8	0.0	1.1	7.225972	43988	45503	1251-2090	1.000000	52.0
psbZ-trnG-UCC_p4	psbZ-trnG-UCC	4	GGTATCCTTAATTCTCTCATCTCTTGA	CGTCTTCTCCTTGGCAAAGA	27	20	37.0	50.0	58.1	57.8	0.0	1.1	7.304743	43988	45503	1251-2090	1.000000	52.0
psbZ-trnG-UCC_p5	psbZ-trnG-UCC	5	ATCCTTAATTCTCTCATCTCTTGA	CGTCTTCTCCTTGGCAAAGA	24	20	33.3	50.0	54.8	57.8	0.0	1.1	7.334848	43988	45503	1248-2087	1.000000	52.0
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	GCGATGCTCTAACCTCTGAG	ACCAATTTCGCCATATCCCC	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.137364	55524	56029	556-636	1.000000	88.0
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	GAGTCGAACCGATGACCATC	ACCAATTTCGCCATATCCCC	20	20	55.0	50.0	58.2	58.0	0.0	0.0	0.163174	55524	56029	587-667	1.000000	87.5
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GCGATGCTCTAACCTCTGAG	TAGCGTCTACCAATTTCGCC	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.202173	55524	56029	564-644	1.000000	87.4
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	GAGTCGAACCGATGACCATC	TAGCGTCTACCAATTTCGCC	20	20	55.0	50.0	58.2	58.1	0.0	0.0	0.227983	55524	56029	595-675	1.000000	86.8
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	AAAAGCCAGCTATCGGAGTC	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	57.7	58.0	0.0	0.0	0.322153	55524	56029	602-682	1.000000	86.0
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	ACGCCCAGAATATATCATATTCGT	AGAAGGTCTACGGTTCGAGT	24	20	37.5	50.0	57.8	57.8	11.4	0.0	4.419321	59656	61891	817-2306	1.000000	56.6
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	AGAAACGCCCAGAATATATCA	AGAAGGTCTACGGTTCGAGT	21	20	38.1	50.0	54.5	57.8	0.0	0.0	4.694969	59656	61891	821-2310	1.000000	54.4
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	ACGCCCAGAATATATCATATTCGT	GAAGGTCTACGGTTCGAGTC	24	20	37.5	55.0	57.8	57.5	11.4	0.0	4.763093	59656	61891	816-2305	1.000000	53.9
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	ACGCCCAGAATATATCATATTCGT	GTTCGAGTCCGTATAGCCCT	24	20	37.5	55.0	57.8	58.7	11.4	0.0	4.905387	59656	61891	805-2294	1.000000	52.8
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	CGCCCAGAATATATCATATTCGT	AGAAGGTCTACGGTTCGAGT	23	20	39.1	50.0	56.2	57.8	11.4	0.0	5.012290	59656	61891	816-2305	1.000000	52.0
accD-psaI_p1	accD-psaI	1	TCCGTTAAAAGGCGTTCTGA	AAAGAAGCCATCGCAATTGC	20	20	45.0	45.0	57.5	58.0	0.0	20.8	0.530210	69456	71174	1172-2918	1.000000	85.7
accD-psaI_p2	accD-psaI	2	TCCGTTAAAAGGCGTTCTGA	TTGCCGGAAATACTAAGCCC	20	20	45.0	50.0	57.5	58.0	0.0	5.0	0.576782	69456	71174	1156-2902	1.000000	85.4
accD-psaI_p3	accD-psaI	3	TCCGTTAAAAGGCGTTCTGA	AAGGCACAAAAATGGAGGGT	20	20	45.0	45.0	57.5	57.9	0.0	0.0	0.631420	69456	71174	1132-2878	1.000000	85.0
accD-psaI_p4	accD-psaI	4	TGAGTTACTTCAGCTCCACG	AAAGAAGCCATCGCAATTGC	20	20	50.0	45.0	57.3	58.0	0.0	20.8	0.725406	69456	71174	1151-2897	1.000000	84.2
accD-psaI_p5	accD-psaI	5	TGAGTTACTTCAGCTCCACG	TTGCCGGAAATACTAAGCCC	20	20	50.0	50.0	57.3	58.0	0.0	5.0	0.771979	69456	71174	1135-2881	1.000000	83.9
petA-psbJ_p1	petA-psbJ	1	GGCCTTTTGTTCTTCTTGGC	ATTCCGGATTGGGTTCATCC	20	20	50.0	50.0	57.8	57.6	0.0	4.1	0.519826	75250	76506	1060-1393	1.000000	85.8
petA-psbJ_p2	petA-psbJ	2	GTTCTTCTTGGCGTCGGTTA	ATTCCGGATTGGGTTCATCC	20	20	50.0	50.0	58.2	57.6	0.0	4.1	0.577960	75250	76506	1052-1385	1.000000	85.4
petA-psbJ_p3	petA-psbJ	3	GCCTTTTGTTCTTCTTGGCG	ATTCCGGATTGGGTTCATCC	20	20	50.0	50.0	58.2	57.6	0.0	4.1	0.594016	75250	76506	1059-1392	1.000000	85.2
petA-psbJ_p4	petA-psbJ	4	CTTGGCGTCGGTTATTTTGG	ATTCCGGATTGGGTTCATCC	20	20	50.0	50.0	57.7	57.6	0.0	4.1	0.621417	75250	76506	1046-1379	1.000000	85.0
petA-psbJ_p5	petA-psbJ	5	TTTTGTTCTTCTTGGCGTCG	ATTCCGGATTGGGTTCATCC	20	20	45.0	50.0	57.3	57.6	0.0	4.1	1.113433	75250	76506	1056-1389	1.000000	81.1
rpl33-rps18_p1	rpl33-rps18	1	GCCTAGTCGATTGGAATTGAGA	TCAATTCGATCCCCCGATTG	22	20	45.5	50.0	57.9	57.7	5.6	0.0	2.314584	80365	80901	371-2108	1.000000	69.0
rpl33-rps18_p2	rpl33-rps18	2	GCCTAGTCGATTGGAATTGAGA	ATTCGATCCCCCGATTGGAT	22	20	45.5	50.0	57.9	58.6	5.6	0.0	2.697814	80365	80901	368-2105	1.000000	65.9
rpl33-rps18_p3	rpl33-rps18	3	GCCTAGTCGATTGGAATTGAG	TCAATTCGATCCCCCGATTG	21	20	47.6	50.0	56.5	57.7	5.6	0.0	2.757534	80365	80901	371-2108	1.000000	65.4
rpl33-rps18_p4	rpl33-rps18	4	GCCTAGTCGATTGGAATTGAGA	CAATTCGATCCCCCGATTGG	22	20	45.5	55.0	57.9	58.8	5.6	0.0	2.821599	80365	80901	370-2107	1.000000	64.9
rpl33-rps18_p5	rpl33-rps18	5	GCCTAGTCGATTGGAATTGAGA	AATTCGATCCCCCGATTGGA	22	20	45.5	50.0	57.9	58.9	5.6	0.0	2.923210	80365	80901	369-2106	1.000000	64.1
clpP-psbB_p1	clpP-psbB	1	CCCAGGATTTCGAAAAGGGA	CGATACCAAGGCAAACCCAT	20	20	50.0	50.0	57.5	58.2	25.0	0.0	0.744699	85300	86165	650-1080	1.000000	85.0
clpP-psbB_p2	clpP-psbB	2	TCCCCAGGATTTCGAAAAGG	CGATACCAAGGCAAACCCAT	20	20	50.0	50.0	57.5	58.2	15.7	0.0	0.744699	85300	86165	652-1082	1.000000	85.0
clpP-psbB_p3	clpP-psbB	3	CCCCAGGATTTCGAAAAGGG	CGATACCAAGGCAAACCCAT	20	20	55.0	50.0	58.5	58.2	23.5	0.0	0.762963	85300	86165	651-1081	1.000000	84.8
clpP-psbB_p4	clpP-psbB	4	CCCAGGATTTCGAAAAGGGA	CAGCAAACGACCAGGATCAT	20	20	50.0	50.0	57.5	58.3	25.0	0.0	0.764646	85300	86165	690-1120	1.000000	84.5
clpP-psbB_p5	clpP-psbB	5	TCCCCAGGATTTCGAAAAGG	CAGCAAACGACCAGGATCAT	20	20	50.0	50.0	57.5	58.3	15.7	0.0	0.764646	85300	86165	692-1122	1.000000	84.5
rpl16-rps3_p1	rpl16-rps3	1	ATCCTTTCCCCGAACCCATA	ACGGAAAAGAAATTGCACGC	20	20	50.0	45.0	58.1	58.2	0.0	0.0	0.332283	95637	98188	2918-2921	0.666667	76.0
rpl16-rps3_p2	rpl16-rps3	2	ATCCTTTCCCCGAACCCATA	GGAAAAGAAATTGCACGCGT	20	20	50.0	45.0	58.1	58.2	0.0	5.6	0.332283	95637	98188	2916-2919	0.666667	76.0
rpl16-rps3_p3	rpl16-rps3	3	GACCTGGTTTAACGACAGCT	GGAAAAGAAATTGCACGCGT	20	20	50.0	45.0	57.8	58.2	0.0	5.6	0.399250	95637	98188	2952-2955	0.666667	75.5
rpl16-rps3_p4	rpl16-rps3	4	CCGAACCCATACGTGTTTCT	ACGGAAAAGAAATTGCACGC	20	20	50.0	45.0	57.9	58.2	0.0	0.0	0.322881	95637	98188	2912-2912	0.333333	50.8
rpl16-rps3_p5	rpl16-rps3	5	CCGAACCCATACGTGTTTCT	GGAAAAGAAATTGCACGCGT	20	20	50.0	45.0	57.9	58.2	0.0	5.6	0.322881	95637	98188	2910-2910	0.333333	50.8
rps3-rps19_p1	rps3-rps19	1	GTCGGAAACCCAGTGGATTT	AAATTTCCGGGGACATGCAA	20	20	50.0	45.0	58.1	58.4	12.0	0.0	0.455833	98846	99597	832-926	1.000000	85.6
rps3-rps19_p2	rps3-rps19	2	GTCGGAAACCCAGTGGATTT	AATTTCCGGGGACATGCAAA	20	20	50.0	45.0	58.1	58.4	12.0	0.0	0.455833	98846	99597	831-925	1.000000	85.6
rps3-rps19_p3	rps3-rps19	3	GTCGGAAACCCAGTGGATTT	ATTTCCGGGGACATGCAAAA	20	20	50.0	45.0	58.1	58.4	12.0	0.0	0.455833	98846	99597	830-924	1.000000	85.6
rps3-rps19_p4	rps3-rps19	4	GTACCAAGTCGGAAACCCAG	ATTTCCGGGGACATGCAAAA	20	20	55.0	45.0	58.2	58.4	0.0	0.0	0.556870	98846	99597	837-931	1.000000	84.7
rps3-rps19_p5	rps3-rps19	5	GTCGGAAACCCAGTGGATTT	GTGGGCCATAAATTGGGAGA	20	20	50.0	50.0	58.1	57.5	12.0	10.7	0.546963	98846	99597	867-961	1.000000	84.5