Hotspot

ycf1

SSC · 107882-113275 · 5394 bp

Back to Spatholobus

Pi 0.0327

MarkerSeek score 56.60

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5394
variable_sites: 176
pi: 0.0327
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TGGTTGGATAATTGGTCACACT	TCTGCCTAAATAATATAAACAGGT	40.9	29.2	57.6	53.5	193 bp	1.0	45.4
ycf1_p2	TGGTTGGATAATTGGTCACACT	TTCTGCCTAAATAATATAAACAGGT	40.9	28.0	57.6	54.3	194 bp	1.0	45.4
ycf1_p3	TGGTTGGATAATTGGTCACAC	TCTGCCTAAATAATATAAACAGGT	42.9	29.2	56.1	53.5	193 bp	1.0	45.4
ycf1_p4	TGGTTGGATAATTGGTCACACT	CTGCCTAAATAATATAAACAGGT	40.9	30.4	57.6	52.0	192 bp	1.0	45.4
ycf1_p5	TGGTTGGATAATTGGTCACAC	TTCTGCCTAAATAATATAAACAGGT	42.9	28.0	56.1	54.3	194 bp	1.0	45.4