Hotspot

psaC-ndhD

SSC · 120077-120197 · 121 bp

Back to Spatholobus

Pi 0.0579

MarkerSeek score 73.00

Primer available yes

Label psaC-ndhD

Feature Metadata

label_name: psaC-ndhD
feature_type: igs
region: SSC
length_bp: 121
variable_sites: 7
pi: 0.0579
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhD_p1	AAACAACTCGAAGCATGGGT	AGGAACCCCCTACTATTGGC	45.0	55.0	58.0	58.5	223-231 bp	1.0	79.5
psaC-ndhD_p2	GGCATGAAACAACTCGAAGC	AGGAACCCCCTACTATTGGC	50.0	55.0	58.0	58.5	229-237 bp	1.0	79.3
psaC-ndhD_p3	AAACAACTCGAAGCATGGGT	GGAACCCCCTACTATTGGCA	45.0	55.0	58.0	58.8	222-230 bp	1.0	77.1
psaC-ndhD_p4	GGCATGAAACAACTCGAAGC	GGAACCCCCTACTATTGGCA	50.0	55.0	58.0	58.8	228-236 bp	1.0	77.0
psaC-ndhD_p5	CAGAGGACTGTGTTGGTTGT	AGGAACCCCCTACTATTGGC	50.0	55.0	57.7	58.5	304-312 bp	1.0	76.1