Hotspot

trnP-UGG-psaJ

LSC · 66252-66468 · 217 bp

Back to Spatholobus

Pi 0.0691

MarkerSeek score 76.20

Primer available yes

Label trnP-UGG-psaJ

Feature Metadata

label_name: trnP-UGG-psaJ
feature_type: igs
region: LSC
length_bp: 217
variable_sites: 15
pi: 0.0691
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-psaJ_p1	ACCCGTGACATTTTGTACCC	TGGTGCCACGGAAAGATATG	50.0	50.0	58.1	58.0	308-323 bp	1.0	82.7
trnP-UGG-psaJ_p2	CCCGTGACATTTTGTACCCA	TGGTGCCACGGAAAGATATG	50.0	50.0	58.1	58.0	307-322 bp	1.0	82.7
trnP-UGG-psaJ_p3	ACCCGTGACATTTTGTACCC	GGTGCCACGGAAAGATATGT	50.0	50.0	58.1	58.0	307-322 bp	1.0	82.7
trnP-UGG-psaJ_p4	CCCGTGACATTTTGTACCCA	GGTGCCACGGAAAGATATGT	50.0	50.0	58.1	58.0	306-321 bp	1.0	82.7
trnP-UGG-psaJ_p5	ACAGGATTTGAACCCGTGAC	TGGTGCCACGGAAAGATATG	50.0	50.0	58.1	58.0	319-334 bp	1.0	82.5