Hotspot

cemA-petA

LSC · 61400-61592 · 193 bp

Back to Spatholobus

Pi 0.0737

MarkerSeek score 73.90

Primer available yes

Label cemA-petA

Feature Metadata

label_name: cemA-petA
feature_type: igs
region: LSC
length_bp: 193
variable_sites: 14
pi: 0.0737
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 73.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
cemA-petA_p1	ACTTGTAGTGATTTATCATTCAATGA	GCATTTGAAATGGGTGCTCG	26.9	50.0	55.3	58.4	328-331 bp	1.0	44.1
cemA-petA_p2	ACTTGTAGTGATTTATCATTCAATG	GCATTTGAAATGGGTGCTCG	28.0	50.0	54.0	58.4	328-331 bp	1.0	44.1
cemA-petA_p3	ACTTGTAGTGATTTATCATTCAATGAA	GCATTTGAAATGGGTGCTCG	25.9	50.0	55.9	58.4	328-331 bp	1.0	44.1
cemA-petA_p4	ACTTGTAGTGATTTATCATTCAATGA	TGCATTTGAAATGGGTGCTC	26.9	45.0	55.3	57.3	329-332 bp	1.0	44.1
cemA-petA_p5	CTTGTAGTGATTTATCATTCAATGA	GCATTTGAAATGGGTGCTCG	28.0	50.0	53.7	58.4	327-330 bp	1.0	44.1