Hotspot

accD-psaI

LSC · 59001-59329 · 329 bp

Back to Spatholobus

Pi 0.0568

MarkerSeek score 77.30

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 329
variable_sites: 18
pi: 0.0568
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 77.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	TGGATATGCCTTCTTTTTCA	CCATTGCAATTGCCGGAAAT	35.0	45.0	52.4	58.0	440 bp	0.5	20.0
accD-psaI_p2	TTGGATATGCCTTCTTTTTCA	CCATTGCAATTGCCGGAAAT	33.3	45.0	53.4	58.0	441 bp	0.5	20.0
accD-psaI_p3	TGGATATGCCTTCTTTTTCA	AAGAAGCCATTGCAATTGCC	35.0	45.0	52.4	57.9	446 bp	0.5	20.0
accD-psaI_p4	TTGGATATGCCTTCTTTTTCA	AAGAAGCCATTGCAATTGCC	33.3	45.0	53.4	57.9	447 bp	0.5	20.0
accD-psaI_p5	TTTGGATATGCCTTCTTTTTCA	CCATTGCAATTGCCGGAAAT	31.8	45.0	54.2	58.0	442 bp	0.5	20.0