Hotspot

psbI-psbK

LSC · 54265-54744 · 480 bp

Back to Spatholobus

Pi 0.039

MarkerSeek score 72.20

Primer available yes

Label psbI-psbK

Feature Metadata

label_name: psbI-psbK
feature_type: igs
region: LSC
length_bp: 480
variable_sites: 18
pi: 0.039
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 72.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-psbK_p1	GGATTACGTCCTGGATCGTT	CTTTTGTTTGGCAAGCTGCT	50.0	45.0	57.1	58.1	592-610 bp	1.0	77.3
psbI-psbK_p2	GGATTACGTCCTGGATCGTT	GTTTGGCAAGCTGCTGTAAG	50.0	50.0	57.1	57.9	587-605 bp	1.0	77.3
psbI-psbK_p3	GGATTACGTCCTGGATCGTT	TGGCAAGCTGCTGTAAGTTT	50.0	45.0	57.1	58.3	584-602 bp	1.0	75.3
psbI-psbK_p4	GGATTACGTCCTGGATCGTT	TTGGCAAGCTGCTGTAAGTT	50.0	45.0	57.1	58.3	585-603 bp	1.0	75.3
psbI-psbK_p5	GGATTACGTCCTGGATCGTT	TTTGGCAAGCTGCTGTAAGT	50.0	45.0	57.1	58.3	586-604 bp	1.0	75.3