Hotspot

trnG-UCC-trnS-GCU

LSC · 53447-53904 · 458 bp

Back to Spatholobus

Pi 0.114

MarkerSeek score 81.40

Primer available yes

Label trnG-UCC-trnS-GCU

Feature Metadata

label_name: trnG-UCC-trnS-GCU
feature_type: igs
region: LSC
length_bp: 458
variable_sites: 50
pi: 0.114
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 81.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnS-GCU_p1	ATGAATCAAACGGGGGATCC	CCACTCAGCCATCTCTCC	50.0	61.1	57.6	56.4	534-555 bp	1.0	58.7
trnG-UCC-trnS-GCU_p2	TAAGGGATCCGATGGAACGA	CCACTCAGCCATCTCTCC	50.0	61.1	57.9	56.4	526 bp	0.5	30.6
trnG-UCC-trnS-GCU_p3	GGGATCCGATGGAACGAATC	CCACTCAGCCATCTCTCC	55.0	61.1	58.2	56.4	523 bp	0.5	29.6
trnG-UCC-trnS-GCU_p4	AGGGATCCGATGGAACGAAT	CCACTCAGCCATCTCTCC	50.0	61.1	58.6	56.4	524 bp	0.5	26.6
trnG-UCC-trnS-GCU_p5	GGATCCGATGGAACGAATCG	CCACTCAGCCATCTCTCC	55.0	61.1	58.6	56.4	522 bp	0.5	26.6