Hotspot

atpI-atpH

LSC · 47267-48518 · 1252 bp

Back to Spatholobus

Pi 0.0347

MarkerSeek score 70.20

Primer available yes

Label atpI-atpH

Feature Metadata

label_name: atpI-atpH
feature_type: igs
region: LSC
length_bp: 1252
variable_sites: 42
pi: 0.0347
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 70.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI-atpH_p1	GCTGCGGCTTATATAGGTGA	AGCAATAACGGAAGCAGCAG	50.0	50.0	57.3	58.9	1330-1429 bp	1.0	71.5
atpI-atpH_p2	GCTGCGGCTTATATAGGTGA	AGCAGCAATAACGGAAGCAG	50.0	50.0	57.3	58.9	1333-1432 bp	1.0	71.5
atpI-atpH_p3	GCTGCGGCTTATATAGGTGA	GCAATAACGGAAGCAGCAGA	50.0	50.0	57.3	58.9	1329-1428 bp	1.0	71.5
atpI-atpH_p4	ACAAGCGGTATTCAAGCTCT	AGCAATAACGGAAGCAGCAG	45.0	50.0	57.2	58.9	1366-1465 bp	1.0	71.2
atpI-atpH_p5	ACAAGCGGTATTCAAGCTCT	AGCAGCAATAACGGAAGCAG	45.0	50.0	57.2	58.9	1369-1468 bp	1.0	71.2