Hotspot

petN-trnC-GCA

LSC · 32525-33603 · 1079 bp

Back to Spatholobus

Pi 0.0393

MarkerSeek score 76.30

Primer available yes

Label petN-trnC-GCA

Feature Metadata

label_name: petN-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1079
variable_sites: 41
pi: 0.0393
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 76.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-trnC-GCA_p1	TTAAAGCAGCCCAAGCAAGA	TTTCCCCAGTTCAAATCCGG	45.0	50.0	58.0	58.1	1146-1148 bp	1.0	84.7
petN-trnC-GCA_p2	CAGCCCAAGCAAGACTTACT	TTTCCCCAGTTCAAATCCGG	50.0	50.0	57.8	58.1	1140-1142 bp	1.0	83.3
petN-trnC-GCA_p3	TTAAAGCAGCCCAAGCAAGA	GGGGGACTGCAAATCCTTTT	45.0	50.0	58.0	58.4	1164-1166 bp	1.0	82.5
petN-trnC-GCA_p4	TCTTCCCCATACTACGAGGG	TTTCCCCAGTTCAAATCCGG	55.0	50.0	57.6	58.1	1190-1192 bp	1.0	81.6
petN-trnC-GCA_p5	CAGCCCAAGCAAGACTTACT	GGGGGACTGCAAATCCTTTT	50.0	50.0	57.8	58.4	1158-1160 bp	1.0	81.0