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psbM-petN

LSC · 31688-32428 · 741 bp

Back to Spatholobus

Pi 0.041

MarkerSeek score 69.00

Primer available yes

Label psbM-petN

Feature Metadata

label_name: psbM-petN
feature_type: igs
region: LSC
length_bp: 741
variable_sites: 30
pi: 0.041
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 69.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-petN_p1	TGTATGCTGCACACCCTTTT	TCTTGCTTGGGCTGCTTTAA	45.0	45.0	58.0	58.0	1434-1442 bp	1.0	82.9
psbM-petN_p2	TGTATGCTGCACACCCTTTT	AGTAAGTCTTGCTTGGGCTG	45.0	50.0	58.0	57.8	1440-1448 bp	1.0	81.4
psbM-petN_p3	ATGTATGCTGCACACCCTTT	TCTTGCTTGGGCTGCTTTAA	45.0	45.0	57.8	58.0	1435-1443 bp	1.0	81.2
psbM-petN_p4	TGTATGCTGCACACCCTTTT	TGGTGTTAGCCCAGAATCCT	45.0	50.0	58.0	58.3	1607-1637 bp	1.0	80.3
psbM-petN_p5	TGTATGCTGCACACCCTTTT	CCCTCGTAGTATGGGGAAGA	45.0	55.0	58.0	57.6	1390-1398 bp	1.0	80.0