Hotspot

trnE-UUC-trnY-GUA

LSC · 30502-30565 · 64 bp

Back to Spatholobus

Pi 0.0781

MarkerSeek score 75.50

Primer available yes

Label trnE-UUC-trnY-GUA

Feature Metadata

label_name: trnE-UUC-trnY-GUA
feature_type: igs
region: LSC
length_bp: 64
variable_sites: 5
pi: 0.0781
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnY-GUA_p1	CTCTCTTTCAAGGAGGCAGC	CCAGCGTAGACATATTGCCA	55.0	50.0	58.3	57.8	175 bp	1.0	78.3
trnE-UUC-trnY-GUA_p2	CTCTCTTTCAAGGAGGCAGC	ACCAGCGTAGACATATTGCC	55.0	50.0	58.3	57.8	176 bp	1.0	78.2
trnE-UUC-trnY-GUA_p3	TCGTCTAGTGGTTCAGGACA	CCAGCGTAGACATATTGCCA	50.0	50.0	57.7	57.8	196 bp	1.0	77.9
trnE-UUC-trnY-GUA_p4	TCGTCTAGTGGTTCAGGACA	ACCAGCGTAGACATATTGCC	50.0	50.0	57.7	57.8	197 bp	1.0	77.9
trnE-UUC-trnY-GUA_p5	CTCTCTTTCAAGGAGGCAGC	CGAATTTACAGTCCGTCCCC	55.0	55.0	58.3	58.4	154 bp	1.0	77.7