Hotspot

psbD-trnT-GGU

LSC · 28148-29548 · 1401 bp

Back to Spatholobus

Pi 0.0476

MarkerSeek score 76.80

Primer available yes

Label psbD-trnT-GGU

Feature Metadata

label_name: psbD-trnT-GGU
feature_type: igs
region: LSC
length_bp: 1401
variable_sites: 64
pi: 0.0476
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 76.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbD-trnT-GGU_p1	AATTTACCAAGAGCTATAGTCA	GGCGTAAGTCATCGGTTCAA	31.8	50.0	52.3	58.3	1432-1460 bp	1.0	46.7
psbD-trnT-GGU_p2	AATTTACCAAGAGCTATAGTCA	GTGGTAGAGTAACGCCATGG	31.8	55.0	52.3	58.1	1455-1483 bp	1.0	46.6
psbD-trnT-GGU_p3	AATTTACCAAGAGCTATAGTCA	TCAGTGGTAGAGTAACGCCA	31.8	50.0	52.3	58.1	1458-1486 bp	1.0	46.6
psbD-trnT-GGU_p4	AAATTTACCAAGAGCTATAGTCA	GTGGTAGAGTAACGCCATGG	30.4	55.0	53.2	58.1	1456-1484 bp	1.0	46.6
psbD-trnT-GGU_p5	AAATTTACCAAGAGCTATAGTCA	TCAGTGGTAGAGTAACGCCA	30.4	50.0	53.2	58.1	1459-1487 bp	1.0	46.6