Hotspot

trnS-GGA-ycf3

LSC · 15623-15880 · 258 bp

Back to Spatholobus

Pi 0.0412

MarkerSeek score 73.20

Primer available yes

Label trnS-GGA-ycf3

Feature Metadata

label_name: trnS-GGA-ycf3
feature_type: igs
region: LSC
length_bp: 258
variable_sites: 10
pi: 0.0412
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 73.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GGA-ycf3_p1	TTCCAATGCTACGCCTTGAA	TTCTCCTGAAGTTGTCGGAA	45.0	45.0	57.8	56.4	377-392 bp	1.0	71.0
trnS-GGA-ycf3_p2	TTCCAATGCTACGCCTTGAA	TTTCTCCTGAAGTTGTCGGA	45.0	45.0	57.8	56.4	378-393 bp	1.0	71.0
trnS-GGA-ycf3_p3	TCGGAAAGAGAGGGATTCGA	TTCTCCTGAAGTTGTCGGAA	50.0	45.0	57.9	56.4	428-443 bp	1.0	70.8
trnS-GGA-ycf3_p4	TCGGAAAGAGAGGGATTCGA	TTTCTCCTGAAGTTGTCGGA	50.0	45.0	57.9	56.4	429-444 bp	1.0	70.8
trnS-GGA-ycf3_p5	TCGGAAAGAGAGGGATTCGA	TCTCCTGAAGTTGTCGGAAT	50.0	45.0	57.9	56.2	427-442 bp	1.0	69.0