Hotspot

ndhB-tRNA-Leu(CAA)

IRa · 152429-153201 · 773 bp

Back to Spathiphyllum

Pi 0.0319

MarkerSeek score 81.20

Primer available yes

Label ndhB-tRNA-Leu(CAA)

Feature Metadata

label_name: ndhB-tRNA-Leu(CAA)
feature_type: igs
region: IRa
length_bp: 773
variable_sites: 23
pi: 0.0319
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 81.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhB-tRNA-Leu(CAA)_p1	TGCAATTGCTCAGGATACCC	GTCTCGCGTGTCTACCATTT	50.0	50.0	57.9	58.0	781-833 bp	1.0	85.8
ndhB-tRNA-Leu(CAA)_p2	TGCAATTGCTCAGGATACCC	CTCGCGTGTCTACCATTTCA	50.0	50.0	57.9	58.0	779-831 bp	1.0	85.8
ndhB-tRNA-Leu(CAA)_p3	TGCAATTGCTCAGGATACCC	GCGTGTCTACCATTTCACCA	50.0	50.0	57.9	58.2	776-828 bp	1.0	84.3
ndhB-tRNA-Leu(CAA)_p4	TGCAATTGCTCAGGATACCC	TCTCGCGTGTCTACCATTTC	50.0	50.0	57.9	57.7	780-832 bp	1.0	83.6
ndhB-tRNA-Leu(CAA)_p5	TGCAATTGCTCAGGATACCC	TCGCGTGTCTACCATTTCAC	50.0	50.0	57.9	58.3	778-830 bp	1.0	83.5