Hotspot

ccsA-ndhD

SSC · 122067-122304 · 238 bp

Back to Spathiphyllum

Pi 0.0622
MarkerSeek score 76.30
Primer available yes
Label ccsA-ndhD

Feature Metadata

label_name
ccsA-ndhD
feature_type
igs
region
SSC
length_bp
238
variable_sites
14
pi
0.0622
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.95
markerseek_score
76.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA-ndhD_p1 AGGACTACATAGTTATGGTTCA TCAGTTGACAAGGTACAAGCT 36.4 42.9 53.7 57.2 280-323 bp 1.0 47.7
ccsA-ndhD_p2 AGGACTACATAGTTATGGTTCATTCA TCAGTTGACAAGGTACAAGCT 34.6 42.9 57.7 57.2 280-323 bp 1.0 47.7
ccsA-ndhD_p3 GGACTACATAGTTATGGTTCATTCA TCAGTTGACAAGGTACAAGCT 36.0 42.9 56.4 57.2 279-322 bp 1.0 47.7
ccsA-ndhD_p4 AGGAATAGGACTACATAGTTATGGT TCAGTTGACAAGGTACAAGCT 36.0 42.9 56.3 57.2 286-329 bp 1.0 47.6
ccsA-ndhD_p5 GGGGTTAATCTATTAGGAATAGGACT TCAGTTGACAAGGTACAAGCT 38.5 42.9 57.3 57.2 300-343 bp 1.0 47.5