Hotspot

psbT-psbN

LSC · 80230-80296 · 67 bp

Back to Spathiphyllum

Pi 0.0746

MarkerSeek score 80.50

Primer available yes

Label psbT-psbN

Feature Metadata

label_name: psbT-psbN
feature_type: igs
region: LSC
length_bp: 67
variable_sites: 5
pi: 0.0746
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-psbN_p1	CGGTCGAATCTATGGAAGCA	TCGCCATCTCCATATCTGGT	50.0	50.0	57.5	58.0	299 bp	1.0	77.1
psbT-psbN_p2	ACGGTCGAATCTATGGAAGC	TCGCCATCTCCATATCTGGT	50.0	50.0	57.5	58.0	300 bp	1.0	77.1
psbT-psbN_p3	CGGTCGAATCTATGGAAGCA	ACCTTAGTCGCCATCTCCAT	50.0	50.0	57.5	58.2	306 bp	1.0	75.6
psbT-psbN_p4	ACGGTCGAATCTATGGAAGC	ACCTTAGTCGCCATCTCCAT	50.0	50.0	57.5	58.2	307 bp	1.0	75.6
psbT-psbN_p5	CGGTCGAATCTATGGAAGCA	GTCGCCATCTCCATATCTGG	50.0	55.0	57.5	57.6	300 bp	1.0	74.2