Hotspot

petA-psbJ

LSC · 68691-69613 · 923 bp

Back to Spathiphyllum

Pi 0.0275

MarkerSeek score 76.10

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 923
variable_sites: 25
pi: 0.0275
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 76.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CGCGTTCAAGGTCTTTTGTT	CTGTGATCGGTTCAATCGGT	45.0	50.0	57.5	58.0	1099-1315 bp	1.0	78.8
petA-psbJ_p2	ACGCGTTCAAGGTCTTTTGT	CTGTGATCGGTTCAATCGGT	45.0	50.0	58.6	58.0	1100-1316 bp	1.0	77.6
petA-psbJ_p3	CGCGTTCAAGGTCTTTTGTT	TCCTGTGATCGGTTCAATCG	45.0	50.0	57.5	57.7	1101-1317 bp	1.0	76.5
petA-psbJ_p4	CGTTACGCGTTCAAGGTCTT	CTGTGATCGGTTCAATCGGT	50.0	50.0	58.9	58.0	1104-1320 bp	1.0	75.6
petA-psbJ_p5	ACGCGTTCAAGGTCTTTTGT	TCCTGTGATCGGTTCAATCG	45.0	50.0	58.6	57.7	1102-1318 bp	1.0	75.3