Hotspot

tRNA-Phe(GAA)-ndhJ

LSC · 52423-53257 · 835 bp

Back to Spathiphyllum

Pi 0.0435

MarkerSeek score 81.70

Primer available yes

Label tRNA-Phe(GAA)-ndhJ

Feature Metadata

label_name: tRNA-Phe(GAA)-ndhJ
feature_type: igs
region: LSC
length_bp: 835
variable_sites: 36
pi: 0.0435
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 81.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tRNA-Phe(GAA)-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	TGAAAGTTGGATAGGCTGGC	50.0	50.0	58.1	57.9	945-1201 bp	1.0	81.8
tRNA-Phe(GAA)-ndhJ_p2	AGTGATGCATGGAAAACGGT	TGAAAGTTGGATAGGCTGGC	45.0	50.0	58.1	57.9	1002-1258 bp	1.0	81.4
tRNA-Phe(GAA)-ndhJ_p3	GTGATGCATGGAAAACGGTC	TGAAAGTTGGATAGGCTGGC	50.0	50.0	57.7	57.9	1001-1257 bp	1.0	80.0
tRNA-Phe(GAA)-ndhJ_p4	CATGGAAAACGGTCGGGATA	TGAAAGTTGGATAGGCTGGC	50.0	50.0	57.7	57.9	995-1251 bp	1.0	79.6
tRNA-Phe(GAA)-ndhJ_p5	CCTCGTGTCACCAGTTCAAA	ATCATCCACGCCTTAAACGT	50.0	45.0	58.1	57.6	976-1232 bp	1.0	79.6