Hotspot

psbK-psbI

LSC · 8772-9261 · 490 bp

Back to Spathiphyllum

Pi 0.146

MarkerSeek score 89.10

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 490
variable_sites: 68
pi: 0.146
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 89.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	GTTTGGCAAGCTGCTGTAAG	CACGCCCAGGATTACGTC	50.0	61.1	57.9	57.9	623-625 bp	1.0	73.9
psbK-psbI_p2	ATGCCCGTCATACCTGTACT	CACGCCCAGGATTACGTC	50.0	61.1	58.2	57.9	662-664 bp	1.0	72.5
psbK-psbI_p3	GTTTGGCAAGCTGCTGTAAG	TCACGCCCAGGATTACGT	50.0	55.6	57.9	58.3	624-626 bp	1.0	72.4
psbK-psbI_p4	TGGCAAGCTGCTGTAAGTTT	CACGCCCAGGATTACGTC	45.0	61.1	58.3	57.9	620-622 bp	1.0	71.9
psbK-psbI_p5	TTGGCAAGCTGCTGTAAGTT	CACGCCCAGGATTACGTC	45.0	61.1	58.3	57.9	621-623 bp	1.0	71.9