Hotspot

ycf1

IRb · 114668-115846 · 1179 bp

Back to Sparganium

Pi 0.0145

MarkerSeek score 58.00

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: IRb
length_bp: 1179
variable_sites: 61
pi: 0.0145
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 58.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CCCGGAACTTCTTCGTAGTG	ATGGGAGGTGGACGAATTTC	55.0	50.0	58.0	57.6	1503-1661 bp	1.0	86.1
ycf1_p2	CCGGAACTTCTTCGTAGTGG	ATGGGAGGTGGACGAATTTC	55.0	50.0	58.0	57.6	1502-1660 bp	1.0	86.1
ycf1_p3	GAGCTACTGAGGAACAACGG	ATGGGAGGTGGACGAATTTC	55.0	50.0	58.0	57.6	1598-1756 bp	1.0	85.8
ycf1_p4	GAGCTACTGAGGAACAACGG	ATCTATGGGAGGTGGACGAA	55.0	50.0	58.0	57.5	1602-1760 bp	1.0	85.4
ycf1_p5	GAGCTACTGAGGAACAACGG	TCTATGGGAGGTGGACGAAT	55.0	50.0	58.0	57.5	1601-1759 bp	1.0	85.4