Hotspot

ycf1-ndhF

SSC · 115847-115981 · 135 bp

Back to Sparganium

Pi 0.104

MarkerSeek score 63.10

Primer available yes

Label ycf1-ndhF

Feature Metadata

label_name: ycf1-ndhF
feature_type: igs
region: SSC
length_bp: 135
variable_sites: 41
pi: 0.104
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 0.73
markerseek_score: 63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-ndhF_p1	ACAGGAACAAGAGGGATCCA	ATGGGAGGTGGACGAATTTC	50.0	50.0	58.0	57.6	470-628 bp	1.0	88.3
ycf1-ndhF_p2	AAGAGGGATCCACCGAAGAA	ATGGGAGGTGGACGAATTTC	50.0	50.0	58.0	57.6	462-620 bp	1.0	88.3
ycf1-ndhF_p3	CGGAAGCCCTTTATTCGGAA	ATGGGAGGTGGACGAATTTC	50.0	50.0	58.0	57.6	426-584 bp	0.941176	86.0
ycf1-ndhF_p4	TTCGGAAGCCCTTTATTCGG	ATGGGAGGTGGACGAATTTC	50.0	50.0	58.0	57.6	428-586 bp	0.941176	86.0
ycf1-ndhF_p5	CGGAAGCCCTTTATTCGGAA	TCTATGGGAGGTGGACGAAT	50.0	50.0	58.0	57.5	429-587 bp	0.941176	85.6