Hotspot

accD-psaI

LSC · 62198-63421 · 1224 bp

Back to Sparganium

Pi 0.0234

MarkerSeek score 69.10

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 1224
variable_sites: 108
pi: 0.0234
unique_haplotype_count: 16
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 69.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	TATTTCAGCTCCACGGCTTC	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.0	58.0	1340-1531 bp	1.0	89.9
accD-psaI_p2	TATTTCAGCTCCACGGCTTC	TTGCTGAGACGGAAATACGG	50.0	50.0	58.0	58.0	1908-2099 bp	1.0	88.8
accD-psaI_p3	TATTTCAGCTCCACGGCTTC	GGTCCCATCGGATCTAGACA	50.0	55.0	58.0	58.0	1394-1453 bp	0.588235	71.2
accD-psaI_p4	TATTTCAGCTCCACGGCTTC	TGGTCCCATCGGATCTAGAC	50.0	55.0	58.0	58.0	1395-1454 bp	0.588235	71.2
accD-psaI_p5	ATTGTGTGTGCTTCTGGAGG	GGTCCCATCGGATCTAGACA	50.0	55.0	58.1	58.0	1743-1802 bp	0.588235	70.1