Hotspot

petN-psbM

LSC · 30290-31536 · 1247 bp

Back to Sparganium

Pi 0.0126

MarkerSeek score 67.60

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1247
variable_sites: 58
pi: 0.0126
unique_haplotype_count: 16
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 67.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCACTGTTCATTCT	50.0	42.9	58.2	57.6	1395-1411 bp	1.0	72.3
petN-psbM_p2	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCACTGTTCATTC	50.0	45.0	58.2	56.1	1395-1411 bp	1.0	68.1
petN-psbM_p3	ATAGTAAGTCTCGCTTGGGC	TGCTACTGCACTGTTCATTCT	50.0	42.9	57.1	57.6	1396-1412 bp	1.0	66.4
petN-psbM_p4	CTCGCTTGGGCTGCTTTAAT	TGCTACTGCACTGTTCATTCT	50.0	42.9	58.9	57.6	1387-1403 bp	1.0	66.3
petN-psbM_p5	TAGTATGGGGAAGGAGTGGG	TGCTACTGCACTGTTCATTCT	55.0	42.9	57.5	57.6	1344-1354 bp	0.941176	66.0