Hotspot

trnS-GCU-trnG-GCC

LSC · 8481-10079 · 1599 bp

Back to Sparganium

Pi 0.0269

MarkerSeek score 71.50

Primer available yes

Label trnS-GCU-trnG-GCC

Feature Metadata

label_name: trnS-GCU-trnG-GCC
feature_type: igs
region: LSC
length_bp: 1599
variable_sites: 154
pi: 0.0269
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 71.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-GCC_p1	ATTAGCAATCCGCCGCTTTA	GGCGTTAATAGAACGAATCACA	45.0	40.9	58.0	56.9	1497-1802 bp	1.0	66.0
trnS-GCU-trnG-GCC_p2	ATTAGCAATCCGCCGCTTTA	GGCGTTAATAGAACGAATCACAC	45.0	43.5	58.0	58.2	1497-1802 bp	1.0	65.5
trnS-GCU-trnG-GCC_p3	ATTAGCAATCCGCCGCTTTA	AGGCGTTAATAGAACGAATCACA	45.0	39.1	58.0	58.2	1498-1803 bp	1.0	64.9
trnS-GCU-trnG-GCC_p4	ATTAGCAATCCGCCGCTTTA	AGGCGTTAATAGAACGAATCAC	45.0	40.9	58.0	56.6	1498-1803 bp	1.0	63.9
trnS-GCU-trnG-GCC_p5	GCTTTAGTCCACTCAGCCAT	GGCGTTAATAGAACGAATCACA	50.0	40.9	57.6	56.9	1483-1788 bp	1.0	63.1