Hotspot

psbK-psbI

LSC · 7716-8148 · 433 bp

Back to Sparganium

Pi 0.0197

MarkerSeek score 63.50

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 433
variable_sites: 35
pi: 0.0197
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 63.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	GTTTGGCAAGCTGCTGTAAG	ACAAAGAGTTTGAGAGTAAGCA	50.0	36.4	57.9	55.6	471-492 bp	1.0	53.3
psbK-psbI_p2	TGGCAAGCTGCTGTAAGTTT	ACAAAGAGTTTGAGAGTAAGCA	45.0	36.4	58.3	55.6	468-489 bp	1.0	51.4
psbK-psbI_p3	TTGGCAAGCTGCTGTAAGTT	ACAAAGAGTTTGAGAGTAAGCA	45.0	36.4	58.3	55.6	469-490 bp	1.0	51.4
psbK-psbI_p4	TTTGGCAAGCTGCTGTAAGT	ACAAAGAGTTTGAGAGTAAGCA	45.0	36.4	58.3	55.6	470-491 bp	1.0	51.3
psbK-psbI_p5	GTTTGGCAAGCTGCTGTAAG	AACAAAGAGTTTGAGAGTAAGCA	50.0	34.8	57.9	56.3	472-493 bp	1.0	51.1