primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	5960	7111	1222-1250	1.000000	83.4
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.2	58.1	0.0	16.9	0.280333	5960	7111	1233-1261	1.000000	82.8
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	AAGTCGCACGTTGCTTTCTA	CTCGGAGGTTCGAATCCTTC	20	20	45.0	55.0	58.1	57.8	8.1	16.9	0.350677	5960	7111	1226-1254	1.000000	82.3
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	TCATGTCCTTCAAGTCGCAC	CTCGGAGGTTCGAATCCTTC	20	20	50.0	55.0	58.2	57.8	0.0	16.9	0.415585	5960	7111	1237-1265	1.000000	81.7
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	AAGTCGCACGTTGCTTTCTA	TACTCGGAGGTTCGAATCCT	20	20	45.0	50.0	58.1	57.6	8.1	16.1	0.571278	5960	7111	1228-1256	1.000000	80.5
psbK-psbI_p1	psbK-psbI	1	GTTTGGCAAGCTGCTGTAAG	ACAAAGAGTTTGAGAGTAAGCA	20	22	50.0	36.4	57.9	55.6	1.4	6.4	4.446983	7716	8148	471-492	1.000000	53.3
psbK-psbI_p2	psbK-psbI	2	TGGCAAGCTGCTGTAAGTTT	ACAAAGAGTTTGAGAGTAAGCA	20	22	45.0	36.4	58.3	55.6	1.4	6.4	4.699693	7716	8148	468-489	1.000000	51.4
psbK-psbI_p3	psbK-psbI	3	TTGGCAAGCTGCTGTAAGTT	ACAAAGAGTTTGAGAGTAAGCA	20	22	45.0	36.4	58.3	55.6	1.4	6.4	4.699693	7716	8148	469-490	1.000000	51.4
psbK-psbI_p4	psbK-psbI	4	TTTGGCAAGCTGCTGTAAGT	ACAAAGAGTTTGAGAGTAAGCA	20	22	45.0	36.4	58.3	55.6	1.4	6.4	4.699693	7716	8148	470-491	1.000000	51.3
psbK-psbI_p5	psbK-psbI	5	GTTTGGCAAGCTGCTGTAAG	AACAAAGAGTTTGAGAGTAAGCA	20	23	50.0	34.8	57.9	56.3	1.4	6.4	4.722713	7716	8148	472-493	1.000000	51.1
trnS-GCU-trnG-GCC_p1	trnS-GCU-trnG-GCC	1	ATTAGCAATCCGCCGCTTTA	GGCGTTAATAGAACGAATCACA	20	22	45.0	40.9	58.0	56.9	0.0	13.6	3.148424	8481	10079	1497-1802	1.000000	66.0
trnS-GCU-trnG-GCC_p2	trnS-GCU-trnG-GCC	2	ATTAGCAATCCGCCGCTTTA	GGCGTTAATAGAACGAATCACAC	20	23	45.0	43.5	58.0	58.2	0.0	13.6	3.219382	8481	10079	1497-1802	1.000000	65.5
trnS-GCU-trnG-GCC_p3	trnS-GCU-trnG-GCC	3	ATTAGCAATCCGCCGCTTTA	AGGCGTTAATAGAACGAATCACA	20	23	45.0	39.1	58.0	58.2	0.0	13.6	3.287146	8481	10079	1498-1803	1.000000	64.9
trnS-GCU-trnG-GCC_p4	trnS-GCU-trnG-GCC	4	ATTAGCAATCCGCCGCTTTA	AGGCGTTAATAGAACGAATCAC	20	22	45.0	40.9	58.0	56.6	0.0	13.6	3.416511	8481	10079	1498-1803	1.000000	63.9
trnS-GCU-trnG-GCC_p5	trnS-GCU-trnG-GCC	5	GCTTTAGTCCACTCAGCCAT	GGCGTTAATAGAACGAATCACA	20	22	50.0	40.9	57.6	56.9	0.9	13.6	3.520166	8481	10079	1483-1788	1.000000	63.1
atpH-atpI_p1	atpH-atpI	1	AACAGAAGCGGCAGAAATCA	CGAATCCATGGAGGGTCATC	20	20	45.0	55.0	58.1	57.8	1.4	21.3	0.279658	14825	15773	983-1011	1.000000	86.8
atpH-atpI_p2	atpH-atpI	2	AGCCAATCCAGCAGCAATAA	CGAATCCATGGAGGGTCATC	20	20	45.0	55.0	57.9	57.8	0.0	21.3	0.324323	14825	15773	1001-1029	1.000000	86.3
atpH-atpI_p3	atpH-atpI	3	CAGCAGCAATAACAGAAGCG	CGAATCCATGGAGGGTCATC	20	20	50.0	55.0	57.8	57.8	0.0	21.3	0.345541	14825	15773	993-1021	1.000000	86.2
atpH-atpI_p4	atpH-atpI	4	GCCAATCCAGCAGCAATAAC	CGAATCCATGGAGGGTCATC	20	20	50.0	55.0	57.8	57.8	0.0	21.3	0.390265	14825	15773	1000-1028	1.000000	85.8
atpH-atpI_p5	atpH-atpI	5	GTCCAATAGAAGCAAGCCCT	CGAATCCATGGAGGGTCATC	20	20	50.0	55.0	57.6	57.8	0.0	21.3	0.602720	14825	15773	1023-1051	1.000000	83.9
petN-psbM_p1	petN-psbM	1	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCACTGTTCATTCT	20	21	50.0	42.9	58.2	57.6	0.0	0.0	1.558772	30290	31536	1395-1411	1.000000	72.3
petN-psbM_p2	petN-psbM	2	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCACTGTTCATTC	20	20	50.0	45.0	58.2	56.1	0.0	0.0	2.081320	30290	31536	1395-1411	1.000000	68.1
petN-psbM_p3	petN-psbM	3	ATAGTAAGTCTCGCTTGGGC	TGCTACTGCACTGTTCATTCT	20	21	50.0	42.9	57.1	57.6	0.0	0.0	2.300208	30290	31536	1396-1412	1.000000	66.4
petN-psbM_p4	petN-psbM	4	CTCGCTTGGGCTGCTTTAAT	TGCTACTGCACTGTTCATTCT	20	21	50.0	42.9	58.9	57.6	0.0	0.0	2.309373	30290	31536	1387-1403	1.000000	66.3
petN-psbM_p5	petN-psbM	5	TAGTATGGGGAAGGAGTGGG	TGCTACTGCACTGTTCATTCT	20	21	55.0	42.9	57.5	57.6	0.0	0.0	1.896951	30290	31536	1344-1354	0.941176	66.0
trnE-UUC-trnT-GGU_p1	trnE-UUC-trnT-GGU	1	CATAGTACCCTACCCCCAGG	GAACCGATGACTTACGCCTT	20	20	60.0	50.0	58.0	58.0	0.0	0.0	0.031606	33463	34227	903-945	1.000000	85.0
trnE-UUC-trnT-GGU_p2	trnE-UUC-trnT-GGU	2	CATAGTACCCTACCCCCAGG	CCATGGCGTTACTCTACCAC	20	20	60.0	55.0	58.0	58.1	0.0	0.0	0.089703	33463	34227	882-924	1.000000	84.6
trnE-UUC-trnT-GGU_p3	trnE-UUC-trnT-GGU	3	CATAGTACCCTACCCCCAGG	TGGCGTTACTCTACCACTGA	20	20	60.0	50.0	58.0	58.1	0.0	0.0	0.111858	33463	34227	879-921	1.000000	84.5
trnE-UUC-trnT-GGU_p4	trnE-UUC-trnT-GGU	4	CATAGTACCCTACCCCCAGG	CCGATGACTTACGCCTTACC	20	20	60.0	55.0	58.0	58.2	0.0	0.0	0.176539	33463	34227	900-942	1.000000	83.8
trnE-UUC-trnT-GGU_p5	trnE-UUC-trnT-GGU	5	GCTGCCTCCTTGAAAGAGAG	GAACCGATGACTTACGCCTT	20	20	55.0	50.0	58.3	58.0	0.0	0.0	0.269359	33463	34227	868-910	1.000000	83.3
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	GCGATGCTCTAACCTCTGAG	ACCAATTTCGCCATATCCCC	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.137364	49033	50116	1090-1152	1.000000	87.5
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	GAGTCGAACCGATGACCATC	ACCAATTTCGCCATATCCCC	20	20	55.0	50.0	58.2	58.0	0.0	0.0	0.163174	49033	50116	1121-1183	1.000000	87.2
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GCGATGCTCTAACCTCTGAG	TAGCGTCTACCAATTTCGCC	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.202173	49033	50116	1098-1160	1.000000	87.0
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	GAGTCGAACCGATGACCATC	TAGCGTCTACCAATTTCGCC	20	20	55.0	50.0	58.2	58.1	0.0	0.0	0.227983	49033	50116	1129-1191	1.000000	86.7
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	TAGAGCAAAAGCCGGCTATC	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	57.8	58.0	33.1	0.0	0.245117	49033	50116	1142-1204	1.000000	86.5
atpB-rbcL_p1	atpB-rbcL	1	CTTCAAGTGTGGAAACCCCA	ACACCAGCTTTAAATCCAGCA	20	21	50.0	42.9	57.9	58.1	0.0	0.0	1.190602	57625	58441	899-931	1.000000	75.5
atpB-rbcL_p2	atpB-rbcL	2	CTTCAAGTGTGGAAACCCCA	CCTTGGTTTCGTACTCAGGAG	20	21	50.0	52.4	57.9	58.0	0.0	0.0	1.105079	57625	58441	957-978	0.941176	71.5
atpB-rbcL_p3	atpB-rbcL	3	TGGGCCAATAATTTGAGCGA	CCTTGGTTTCGTACTCAGGAG	20	21	45.0	52.4	57.9	58.0	2.1	0.0	1.175479	57625	58441	995-1016	0.941176	70.8
atpB-rbcL_p4	atpB-rbcL	4	CTTCAAGTGTGGAAACCCCA	CCTTGGTTTCGTACTCAGGA	20	20	50.0	50.0	57.9	56.9	0.0	0.0	1.187872	57625	58441	957-978	0.941176	70.8
atpB-rbcL_p5	atpB-rbcL	5	CTTCAAGTGTGGAAACCCCA	TCCTTGGTTTCGTACTCAGG	20	20	50.0	50.0	57.9	56.9	0.0	0.0	1.187872	57625	58441	958-979	0.941176	70.8
accD-psaI_p1	accD-psaI	1	TATTTCAGCTCCACGGCTTC	CCATTGCAATTGCCGGAAAT	20	20	50.0	45.0	58.0	58.0	0.0	26.9	0.051179	62198	63421	1340-1531	1.000000	89.9
accD-psaI_p2	accD-psaI	2	TATTTCAGCTCCACGGCTTC	TTGCTGAGACGGAAATACGG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.028758	62198	63421	1908-2099	1.000000	88.8
accD-psaI_p3	accD-psaI	3	TATTTCAGCTCCACGGCTTC	GGTCCCATCGGATCTAGACA	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.027377	62198	63421	1394-1453	0.588235	71.2
accD-psaI_p4	accD-psaI	4	TATTTCAGCTCCACGGCTTC	TGGTCCCATCGGATCTAGAC	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.027377	62198	63421	1395-1454	0.588235	71.2
accD-psaI_p5	accD-psaI	5	ATTGTGTGTGCTTCTGGAGG	GGTCCCATCGGATCTAGACA	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.101611	62198	63421	1743-1802	0.588235	70.1
petA-psbJ_p1	petA-psbJ	1	CAGGGCCAGAACTTCTTGTT	TCCCTATTCATTGCGGGTTG	20	20	50.0	50.0	58.0	57.9	6.2	0.0	0.064452	67176	68318	2052-2077	1.000000	84.2
petA-psbJ_p2	petA-psbJ	2	CAGGGCCAGAACTTCTTGTT	GGGTTAGCTTACGACGTGTT	20	20	50.0	50.0	58.0	57.9	6.2	11.6	0.083379	67176	68318	2016-2041	1.000000	84.1
petA-psbJ_p3	petA-psbJ	3	CAGGGCCAGAACTTCTTGTT	ACGGACTAGCTGTACCTACC	20	20	50.0	55.0	58.0	58.0	6.2	0.0	0.063006	67176	68318	1816-1841	1.000000	84.0
petA-psbJ_p4	petA-psbJ	4	CAGGGCCAGAACTTCTTGTT	GGACTAGCTGTACCTACCGT	20	20	50.0	55.0	58.0	58.0	6.2	0.0	0.063006	67176	68318	1814-1839	1.000000	84.0
petA-psbJ_p5	petA-psbJ	5	TAGGTGGGAACAGAGGAAGG	GGACTAGCTGTACCTACCGT	20	20	55.0	55.0	58.0	58.0	0.0	0.0	0.097267	67176	68318	1991-2016	0.941176	81.1
trnW-CCA-trnP-UGG_p1	trnW-CCA-trnP-UGG	1	GCGTTCTACCGAACTGAACT	GTCACGGGTTCAAATCCTGT	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.169530	71105	71255	206-209	1.000000	86.4
trnW-CCA-trnP-UGG_p2	trnW-CCA-trnP-UGG	2	GCGTTCTACCGAACTGAACT	CACGGGTTCAAATCCTGTCA	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.170864	71105	71255	204-207	1.000000	86.4
trnW-CCA-trnP-UGG_p3	trnW-CCA-trnP-UGG	3	GCGTTCTACCGAACTGAACT	TGTCACGGGTTCAAATCCTG	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.170864	71105	71255	207-210	1.000000	86.3
trnW-CCA-trnP-UGG_p4	trnW-CCA-trnP-UGG	4	GCGTTCTACCGAACTGAACT	GGGTACAAAATGTCACGGGT	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.161494	71105	71255	217-220	1.000000	86.1
trnW-CCA-trnP-UGG_p5	trnW-CCA-trnP-UGG	5	GCGTTCTACCGAACTGAACT	TGGGTACAAAATGTCACGGG	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.162838	71105	71255	218-221	1.000000	86.0
petD-rpoA_p1	petD-rpoA	1	GTTGTAGTTGCAACTCCGGA	GCGGATATCGTATGGAACCC	20	20	50.0	55.0	58.1	58.0	34.3	0.0	0.132273	82543	82757	1436-1517	1.000000	84.3
petD-rpoA_p2	petD-rpoA	2	CTCGAGCCGGATGATGAAAA	GCGGATATCGTATGGAACCC	20	20	50.0	55.0	58.1	58.0	5.0	0.0	0.065996	82543	82757	1306-1326	0.941176	80.4
petD-rpoA_p3	petD-rpoA	3	CTCGAGCCGGATGATGAAAA	GGTCCGAGATATGTAACCGC	20	20	50.0	55.0	58.1	57.9	5.0	0.0	0.121765	82543	82757	1436-1456	0.941176	79.9
petD-rpoA_p4	petD-rpoA	4	CTCGAGCCGGATGATGAAAA	TAGCTAACCTGACGGAACCA	20	20	50.0	50.0	58.1	58.1	5.0	0.0	0.132538	82543	82757	1363-1383	0.941176	79.9
petD-rpoA_p5	petD-rpoA	5	CTCGAGCCGGATGATGAAAA	ACCGTCCGTGGAAATAGTTG	20	20	50.0	50.0	58.1	57.9	5.0	0.0	0.148975	82543	82757	1398-1418	0.941176	79.7
rpoA-rps11_p1	rpoA-rps11	1	TGCTACTTCCTCTCGAACCA	ACGAGATGCAGCATTACGAG	20	20	50.0	50.0	58.1	58.2	0.0	28.9	0.235494	83778	83845	197-204	1.000000	85.3
rpoA-rps11_p2	rpoA-rps11	2	TGCTACTTCCTCTCGAACCA	GACGAGATGCAGCATTACGA	20	20	50.0	50.0	58.1	58.2	0.0	28.9	0.240579	83778	83845	198-205	1.000000	85.2
rpoA-rps11_p3	rpoA-rps11	3	ATTGTTCGAGTGGATCCTGC	ACGAGATGCAGCATTACGAG	20	20	50.0	50.0	58.0	58.2	14.2	28.9	0.184572	83778	83845	214-221	1.000000	85.1
rpoA-rps11_p4	rpoA-rps11	4	ATTGTTCGAGTGGATCCTGC	GACGAGATGCAGCATTACGA	20	20	50.0	50.0	58.0	58.2	14.2	28.9	0.189658	83778	83845	215-222	1.000000	85.0
rpoA-rps11_p5	rpoA-rps11	5	TCGAGTGGATCCTGCTACTT	ACGAGATGCAGCATTACGAG	20	20	50.0	50.0	57.9	58.2	14.2	28.9	0.300131	83778	83845	209-216	1.000000	84.3
ycf1_p1	ycf1	1	CCCGGAACTTCTTCGTAGTG	ATGGGAGGTGGACGAATTTC	20	20	55.0	50.0	58.0	57.6	0.7	0.0	0.427889	114668	115846	1503-1661	1.000000	86.1
ycf1_p2	ycf1	2	CCGGAACTTCTTCGTAGTGG	ATGGGAGGTGGACGAATTTC	20	20	55.0	50.0	58.0	57.6	0.0	0.0	0.427889	114668	115846	1502-1660	1.000000	86.1
ycf1_p3	ycf1	3	GAGCTACTGAGGAACAACGG	ATGGGAGGTGGACGAATTTC	20	20	55.0	50.0	58.0	57.6	0.0	0.0	0.422389	114668	115846	1598-1756	1.000000	85.8
ycf1_p4	ycf1	4	GAGCTACTGAGGAACAACGG	ATCTATGGGAGGTGGACGAA	20	20	55.0	50.0	58.0	57.5	0.0	0.0	0.474146	114668	115846	1602-1760	1.000000	85.4
ycf1_p5	ycf1	5	GAGCTACTGAGGAACAACGG	TCTATGGGAGGTGGACGAAT	20	20	55.0	50.0	58.0	57.5	0.0	0.0	0.474146	114668	115846	1601-1759	1.000000	85.4
ycf1-ndhF_p1	ycf1-ndhF	1	ACAGGAACAAGAGGGATCCA	ATGGGAGGTGGACGAATTTC	20	20	50.0	50.0	58.0	57.6	15.2	0.0	0.464851	115847	115981	470-628	1.000000	88.3
ycf1-ndhF_p2	ycf1-ndhF	2	AAGAGGGATCCACCGAAGAA	ATGGGAGGTGGACGAATTTC	20	20	50.0	50.0	58.0	57.6	15.2	0.0	0.467437	115847	115981	462-620	1.000000	88.3
ycf1-ndhF_p3	ycf1-ndhF	3	CGGAAGCCCTTTATTCGGAA	ATGGGAGGTGGACGAATTTC	20	20	50.0	50.0	58.0	57.6	0.0	0.0	0.452739	115847	115981	426-584	0.941176	86.0
ycf1-ndhF_p4	ycf1-ndhF	4	TTCGGAAGCCCTTTATTCGG	ATGGGAGGTGGACGAATTTC	20	20	50.0	50.0	58.0	57.6	0.0	0.0	0.452739	115847	115981	428-586	0.941176	86.0
ycf1-ndhF_p5	ycf1-ndhF	5	CGGAAGCCCTTTATTCGGAA	TCTATGGGAGGTGGACGAAT	20	20	50.0	50.0	58.0	57.5	0.0	0.0	0.504496	115847	115981	429-587	0.941176	85.6
ndhF-rpl32_p1	ndhF-rpl32	1	AGAAAAGGTATTATCCATGCA	TTTGACATAGAAGTACGTTTTT	21	22	33.3	27.3	52.6	52.0	0.0	0.0	14.408967	118199	119152	1022-1067	1.000000	49.5
ndhF-rpl32_p2	ndhF-rpl32	2	AGAAAAGGTATTATCCATGCA	TTTTGACATAGAAGTACGTTTT	21	22	33.3	27.3	52.6	52.0	0.0	0.0	14.408967	118199	119152	1023-1068	1.000000	49.4
ndhF-rpl32_p3	ndhF-rpl32	3	AGAAAAGGTATTATCCATGCA	TTTTTGACATAGAAGTACGTTT	21	22	33.3	27.3	52.6	52.0	0.0	0.0	14.408967	118199	119152	1024-1069	1.000000	49.4
ndhF-rpl32_p4	ndhF-rpl32	4	AGAAAAGGTATTATCCATGCA	TCTTCCAAATATTTCTACGAATACG	21	25	33.3	32.0	52.6	55.4	0.0	0.0	14.095870	118199	119152	1051-1096	1.000000	49.2
ndhF-rpl32_p5	ndhF-rpl32	5	AGAAAAGGTATTATCCATGCAT	TCTTCCAAATATTTCTACGAATACG	22	25	31.8	32.0	53.2	55.4	0.0	0.0	14.412779	118199	119152	1051-1096	1.000000	49.2
psaC-ndhE_p1	psaC-ndhE	1	ATCGAGTACGGGCTTTTGTC	CTGCTGAAGCTGCTATTGGA	20	20	50.0	50.0	58.0	58.0	0.0	0.9	0.040075	123386	124182	1033-1321	0.941176	88.3
psaC-ndhE_p2	psaC-ndhE	2	ATCGAGTACGGGCTTTTGTC	GCTGAAGCTGCTATTGGACT	20	20	50.0	50.0	58.0	58.0	0.0	0.9	0.041501	123386	124182	1031-1319	0.941176	88.3
psaC-ndhE_p3	psaC-ndhE	3	ATTCCCAGTCTCCAGTGGAA	CTGCTGAAGCTGCTATTGGA	20	20	50.0	50.0	58.0	58.0	14.2	0.9	0.078897	123386	124182	1334-1622	0.941176	87.3
psaC-ndhE_p4	psaC-ndhE	4	TCCCAGTCTCCAGTGGAAAT	CTGCTGAAGCTGCTATTGGA	20	20	50.0	50.0	58.0	58.0	14.2	0.9	0.078897	123386	124182	1332-1620	0.941176	87.3
psaC-ndhE_p5	psaC-ndhE	5	TCCCAGTCTCCAGTGGAAAT	GCTGAAGCTGCTATTGGACT	20	20	50.0	50.0	58.0	58.0	14.2	0.9	0.080323	123386	124182	1330-1618	0.941176	87.3