Hotspot

SobiCr001

IRb · 96034-97525 · 1492 bp

Back to Sorghum

Pi 0.000223

MarkerSeek score 43.30

Primer available yes

Label SobiCr001

Feature Metadata

label_name: SobiCr001
feature_type: rRNA
region: IRb
length_bp: 1492
variable_sites: 1
pi: 0.000223
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
SobiCr001_p1	CGAATCCGCTTTGTCTACGA	CAGACGTAGCTGCCTTCTTT	50.0	50.0	58.1	57.9	1623 bp	1.0	78.6
SobiCr001_p2	TCCGAATCCGCTTTGTCTAC	CAGACGTAGCTGCCTTCTTT	50.0	50.0	57.7	57.9	1625 bp	1.0	77.0
SobiCr001_p3	AAGACAATTCCGAATCCGCT	CAGACGTAGCTGCCTTCTTT	45.0	50.0	57.6	57.9	1633 bp	1.0	76.1
SobiCr001_p4	AGACAATTCCGAATCCGCTT	CAGACGTAGCTGCCTTCTTT	45.0	50.0	57.6	57.9	1632 bp	1.0	76.1
SobiCr001_p5	ATTCCGAATCCGCTTTGTCT	CAGACGTAGCTGCCTTCTTT	45.0	50.0	57.6	57.9	1627 bp	1.0	76.1