Hotspot

trnS-GGA-rps4

LSC · 47659-47938 · 280 bp

Back to Sorghum

Pi 0.00571

MarkerSeek score 37.70

Primer available yes

Label trnS-GGA-rps4

Feature Metadata

label_name: trnS-GGA-rps4
feature_type: igs
region: LSC
length_bp: 280
variable_sites: 3
pi: 0.00571
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 37.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GGA-rps4_p1	ATGTAGGAGAGATGGCCGAG	AGATAGGAAGTGGGTCGGTC	55.0	55.0	58.4	58.2	442 bp	1.0	75.8
trnS-GGA-rps4_p2	ATGTAGGAGAGATGGCCGAG	ATAGGAAGTGGGTCGGTCTC	55.0	55.0	58.4	58.2	440 bp	1.0	75.8
trnS-GGA-rps4_p3	ATGTAGGAGAGATGGCCGAG	GATAGGAAGTGGGTCGGTCT	55.0	55.0	58.4	58.2	441 bp	1.0	75.8
trnS-GGA-rps4_p4	ATGTAGGAGAGATGGCCGAG	GAAGTGGGTCGGTCTCAAAA	55.0	50.0	58.4	57.8	436 bp	1.0	75.6
trnS-GGA-rps4_p5	ATGTAGGAGAGATGGCCGAG	TTTGACGGTTGACACATTGC	55.0	45.0	58.4	57.5	490 bp	1.0	73.6