Hotspot

trnC-GCA-rpoB

LSC · 21059-22261 · 1203 bp

Back to Sorghum

Pi 0.000887

MarkerSeek score 47.10

Primer available yes

Label trnC-GCA-rpoB

Feature Metadata

label_name: trnC-GCA-rpoB
feature_type: igs
region: LSC
length_bp: 1203
variable_sites: 2
pi: 0.000887
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-rpoB_p1	AAAGGATTTGCAGTCCCCTG	CTTCTCAAGTTCTTCCGCCA	50.0	50.0	58.1	57.8	1355-1359 bp	1.0	78.3
trnC-GCA-rpoB_p2	AAAGGATTTGCAGTCCCCTG	ACTTCTCAAGTTCTTCCGCC	50.0	50.0	58.1	57.8	1356-1360 bp	1.0	78.3
trnC-GCA-rpoB_p3	AAAGGATTTGCAGTCCCCTG	AGTTCTTCCGCCAATCCTTG	50.0	50.0	58.1	58.2	1348-1352 bp	1.0	78.3
trnC-GCA-rpoB_p4	AAAGGATTTGCAGTCCCCTG	CAAGTTCTTCCGCCAATCCT	50.0	50.0	58.1	58.2	1350-1354 bp	1.0	78.3
trnC-GCA-rpoB_p5	AAAGGATTTGCAGTCCCCTG	GTTCTTCCGCCAATCCTTGA	50.0	50.0	58.1	58.2	1347-1351 bp	1.0	78.2