Hotspot

rps16

LSC · 5213-6352 · 1140 bp

Back to Sorbus

Pi 0.00317
MarkerSeek score 57.80
Primer available yes
Label rps16

Feature Metadata

label_name
rps16
feature_type
gene
region
LSC
length_bp
1140
variable_sites
31
pi
0.00317
unique_haplotype_count
46
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16_p1 AGAAACAAAAAGGTTATACAAGGCT TATGTACCGACCGAACCGAT 32.0 50.0 57.4 58.3 1270-1284 bp 1.0 43.3
rps16_p2 AGAAACAAAAAGGTTATACAAGGCT CCGACCGAACCGATGATTAT 32.0 50.0 57.4 57.6 1264-1278 bp 1.0 43.3
rps16_p3 ACAAAAAGGTTATACAAGGCT TATGTACCGACCGAACCGAT 33.3 50.0 53.3 58.3 1266-1280 bp 1.0 43.3
rps16_p4 AGAAACAAAAAGGTTATACAAGGCT ATTATGTACCGACCGAACCG 32.0 50.0 57.4 57.5 1272-1286 bp 1.0 43.3
rps16_p5 GGAAATAGCAAAGAAACAAAAAGGT TATGTACCGACCGAACCGAT 32.0 50.0 57.2 58.3 1281-1295 bp 0.961538 41.7