Hotspot

ndhF-rpl32

SSC · 116517-117566 · 1050 bp

Back to Sorbus

Pi 0.00687
MarkerSeek score 65.40
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
1050
variable_sites
57
pi
0.00687
unique_haplotype_count
38
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 TTGTTGCCGTTGGAAAAAGT AAAAGCTTTCAATGCTGCCC 40.0 45.0 57.0 57.8 1237-1319 bp 1.0 76.6
ndhF-rpl32_p2 TTGTTGCCGTTGGAAAAAGT AAGCTTTCAATGCTGCCCTA 40.0 45.0 57.0 57.8 1235-1317 bp 1.0 76.2
ndhF-rpl32_p3 TTGTTGCCGTTGGAAAAAGT GCTGCCCTATATCCCTTCCT 40.0 55.0 57.0 58.3 1224-1306 bp 1.0 75.3
ndhF-rpl32_p4 TTGTTGCCGTTGGAAAAAGT AGCTTTCAATGCTGCCCTAT 40.0 45.0 57.0 57.6 1234-1316 bp 1.0 74.4
ndhF-rpl32_p5 TTTGTTGCCGTTGGAAAAAGT AAAAGCTTTCAATGCTGCCC 38.1 45.0 57.7 57.8 1238-1320 bp 1.0 74.3