Hotspot

rpl22-rps19

LSC · 87670-87735 · 66 bp

Back to Sorbus

Pi 0.0178
MarkerSeek score 61.30
Primer available yes
Label rpl22-rps19

Feature Metadata

label_name
rpl22-rps19
feature_type
igs
region
LSC
length_bp
66
variable_sites
7
pi
0.0178
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl22-rps19_p1 CGCCCACGAATTTGATCAAT TCTAAATTTCCGGGGGCATG 45.0 50.0 57.2 57.9 220-297 bp 1.0 77.5
rpl22-rps19_p2 CGCCCACGAATTTGATCAAT CTCTAAATTTCCGGGGGCAT 45.0 50.0 57.2 57.6 221-298 bp 1.0 75.1
rpl22-rps19_p3 CGCCCACGAATTTGATCAATT TCTAAATTTCCGGGGGCATG 42.9 50.0 57.9 57.9 220-297 bp 1.0 75.1
rpl22-rps19_p4 CCGCCCACGAATTTGATCAA TCTAAATTTCCGGGGGCATG 50.0 50.0 59.2 57.9 221-298 bp 1.0 74.8
rpl22-rps19_p5 CGCCCACGAATTTGATCAAT ACGGATCGTATGGTAGGACA 45.0 50.0 57.2 57.6 264-341 bp 1.0 74.4