Hotspot

trnF-GAA-ndhJ

LSC · 51575-52276 · 702 bp

Back to Sorbus

Pi 0.00328
MarkerSeek score 56.20
Primer available yes
Label trnF-GAA-ndhJ

Feature Metadata

label_name
trnF-GAA-ndhJ
feature_type
igs
region
LSC
length_bp
702
variable_sites
21
pi
0.00328
unique_haplotype_count
28
primer_available
yes
alignment_reliability
1.00
markerseek_score
56.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnF-GAA-ndhJ_p1 GGGATAGCTCAGCTGGTAGA ATCCACGCCTGAAACGTATC 55.0 50.0 58.0 58.1 866-923 bp 1.0 83.4
trnF-GAA-ndhJ_p2 CCTCGTGTCACCAGTTCAAA ATCCACGCCTGAAACGTATC 50.0 50.0 58.1 58.1 830-887 bp 1.0 83.2
trnF-GAA-ndhJ_p3 CTGGTAGAGCAGAGGACTGA ATCCACGCCTGAAACGTATC 55.0 50.0 57.9 58.1 854-911 bp 1.0 82.4
trnF-GAA-ndhJ_p4 GTCGTGAATGGTCGGGATAG ATCCACGCCTGAAACGTATC 55.0 50.0 57.9 58.1 879-936 bp 1.0 82.3
trnF-GAA-ndhJ_p5 GAGGATGATGCGTCGTGAAT ATCCACGCCTGAAACGTATC 50.0 50.0 58.1 58.1 890-947 bp 1.0 82.3