Hotspot

petN-psbM

LSC · 30152-31396 · 1245 bp

Back to Sorbus

Pi 0.00949
MarkerSeek score 49.20
Primer available yes
Label petN-psbM

Feature Metadata

label_name
petN-psbM
feature_type
igs
region
LSC
length_bp
1245
variable_sites
59
pi
0.00949
unique_haplotype_count
26
primer_available
yes
alignment_reliability
1.00
markerseek_score
49.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-psbM_p1 AGTATGGGGAAGGAGTGGAC AAAACAGTCAGCCAAGGTGA 55.0 45.0 58.1 57.6 1182-1337 bp 1.0 83.2
petN-psbM_p2 GTATGGGGAAGGAGTGGACT AAAACAGTCAGCCAAGGTGA 55.0 45.0 58.1 57.6 1181-1336 bp 1.0 83.2
petN-psbM_p3 TATGGGGAAGGAGTGGACTC AAAACAGTCAGCCAAGGTGA 55.0 45.0 57.8 57.6 1180-1335 bp 1.0 82.8
petN-psbM_p4 GGGGAAGGAGTGGACTCTAG AAAACAGTCAGCCAAGGTGA 60.0 45.0 58.2 57.6 1177-1332 bp 1.0 82.5
petN-psbM_p5 GTATGGGGAAGGAGTGGACT AACAGTCAGCCAAGGTGATT 55.0 45.0 58.1 57.3 1179-1334 bp 1.0 81.4