Hotspot

trnR-UCU-atpA

LSC · 10089-10763 · 675 bp

Back to Sorbus

Pi 0.0314
MarkerSeek score 84.30
Primer available yes
Label trnR-UCU-atpA

Feature Metadata

label_name
trnR-UCU-atpA
feature_type
igs
region
LSC
length_bp
675
variable_sites
140
pi
0.0314
unique_haplotype_count
43
primer_available
yes
alignment_reliability
0.99
markerseek_score
84.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-UCU-atpA_p1 TGGAATGAAAAGCGTCCATTG AGACATTTACCGACGAAGCG 42.9 50.0 57.4 58.4 776-1335 bp 0.961538 74.9
trnR-UCU-atpA_p2 GGAATGAAAAGCGTCCATTGT AGACATTTACCGACGAAGCG 42.9 50.0 57.4 58.4 775-1334 bp 0.961538 74.9
trnR-UCU-atpA_p3 GGTTCAAATCCTATTGGACGC AGACATTTACCGACGAAGCG 47.6 50.0 57.3 58.4 715-1274 bp 0.961538 73.9
trnR-UCU-atpA_p4 TGGAATGAAAAGCGTCCATT AGACATTTACCGACGAAGCG 40.0 50.0 55.9 58.4 776-1335 bp 0.961538 70.8
trnR-UCU-atpA_p5 TTGGAATGAAAAGCGTCCAT AGACATTTACCGACGAAGCG 40.0 50.0 55.9 58.4 777-1336 bp 0.961538 70.8