Hotspot

rps18

LSC · 72123-72434 · 312 bp

Back to Sorbaria

Pi 0.0256

MarkerSeek score 42.70

Primer available yes

Label rps18

Feature Metadata

label_name: rps18
feature_type: gene
region: LSC
length_bp: 312
variable_sites: 12
pi: 0.0256
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 42.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps18_p1	GGTTCATGGCCAAGGGTAAA	CGTTTGTGGATCACTCGGAT	50.0	50.0	58.1	58.0	1151-1165 bp	1.0	86.1
rps18_p2	TTCTTCCTTGCTTCGGATCG	CGTTTGTGGATCACTCGGAT	50.0	50.0	58.0	58.0	1222 bp	0.666667	66.6
rps18_p3	TTCTTCCTTGCTTCGGATCG	GGACACGGGAAGGGAATAAC	50.0	55.0	58.0	58.0	1464 bp	0.666667	66.4
rps18_p4	TTCTTCCTTGCTTCGGATCG	AGGACACGGGAAGGGAATAA	50.0	50.0	58.0	58.0	1465 bp	0.666667	66.4
rps18_p5	TTCTTCCTTGCTTCGGATCG	TCGTCGTTTGTGGATCACTC	50.0	50.0	58.0	57.9	1226 bp	0.666667	66.2