Hotspot

rps16

LSC · 5656-6801 · 1146 bp

Back to Sorbaria

Pi 0.041

MarkerSeek score 72.10

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1146
variable_sites: 70
pi: 0.041
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	ACTTGTGTTGGATTGGCACT	TATGTACCGACCGAACCGAT	45.0	50.0	57.9	58.3	1679-1685 bp	0.666667	63.9
rps16_p2	ACTTGTGTTGGATTGGCACT	CCGACCGAACCGATGATTAT	45.0	50.0	57.9	57.6	1673-1679 bp	0.666667	63.0
rps16_p3	AGGACTTGTGTTGGATTGGC	TATGTACCGACCGAACCGAT	50.0	50.0	58.4	58.3	1682-1688 bp	0.666667	61.4
rps16_p4	GACTTGTGTTGGATTGGCAC	TATGTACCGACCGAACCGAT	50.0	50.0	57.6	58.3	1680-1686 bp	0.666667	61.1
rps16_p5	AGGACTTGTGTTGGATTGGC	CCGACCGAACCGATGATTAT	50.0	50.0	58.4	57.6	1676-1682 bp	0.666667	60.5