Hotspot

psaJ-rpl33

LSC · 71245-71724 · 480 bp

Back to Sorbaria

Pi 0.0583

MarkerSeek score 81.30

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 480
variable_sites: 42
pi: 0.0583
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 81.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	TTTTTCCGGATGCGCTGATA	ACATCTTTACCCTTGGCCAT	45.0	45.0	58.0	56.8	505-541 bp	1.0	80.3
psaJ-rpl33_p2	GATGCGCTGATATTCCCCTT	ACATCTTTACCCTTGGCCAT	50.0	45.0	57.8	56.8	497-533 bp	1.0	79.1
psaJ-rpl33_p3	TTCCGGATGCGCTGATATTC	ACATCTTTACCCTTGGCCAT	50.0	45.0	58.2	56.8	502-538 bp	1.0	79.0
psaJ-rpl33_p4	GTTCGGGTCTTTAGCAGGTT	ACATCTTTACCCTTGGCCAT	50.0	45.0	57.8	56.8	546-582 bp	1.0	78.6
psaJ-rpl33_p5	TGGCACCGGTAGTAAGTACT	ACATCTTTACCCTTGGCCAT	50.0	45.0	57.8	56.8	571-607 bp	1.0	78.1