Hotspot

ycf4-cemA

LSC · 64359-64928 · 570 bp

Back to Sorbaria

Pi 0.0442

MarkerSeek score 75.10

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 570
variable_sites: 37
pi: 0.0442
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 75.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	GCGGGTTCTCGAAAAACAAG	TCCACCAAGGCAAAAACACT	50.0	45.0	58.0	58.1	1148-1153 bp	0.666667	65.8
ycf4-cemA_p2	TGCGCGTACCAATTGAAGTA	TCCACCAAGGCAAAAACACT	45.0	45.0	57.9	58.1	658-663 bp	0.666667	65.7
ycf4-cemA_p3	CGTTGGGGATTTCCTGGAAA	TCCACCAAGGCAAAAACACT	50.0	45.0	58.1	58.1	872-877 bp	0.666667	65.5
ycf4-cemA_p4	TTTCGTTGGGGATTTCCTGG	TCCACCAAGGCAAAAACACT	50.0	45.0	58.1	58.1	875-880 bp	0.666667	65.5
ycf4-cemA_p5	TCTATGGGATCGCAGGTCTT	TCCACCAAGGCAAAAACACT	50.0	45.0	57.9	58.1	985-990 bp	0.666667	65.4