Hotspot

psaI-ycf4

LSC · 63383-63803 · 421 bp

Back to Sorbaria

Pi 0.0475

MarkerSeek score 74.40

Primer available yes

Label psaI-ycf4

Feature Metadata

label_name: psaI-ycf4
feature_type: igs
region: LSC
length_bp: 421
variable_sites: 30
pi: 0.0475
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaI-ycf4_p1	ATTTCCGGCAATTGCAATGG	TACGTTCTGATCGCCAACTC	45.0	50.0	58.0	57.7	504-544 bp	1.0	84.7
psaI-ycf4_p2	GGGCTTAGTATTTCCGGCAA	TACGTTCTGATCGCCAACTC	50.0	50.0	58.0	57.7	513-553 bp	1.0	84.4
psaI-ycf4_p3	GGCAATTGCAATGGCTTCTT	TACGTTCTGATCGCCAACTC	45.0	50.0	57.9	57.7	498-538 bp	1.0	84.2
psaI-ycf4_p4	ATTTCCGGCAATTGCAATGG	CGTTCTGATCGCCAACTCAT	45.0	50.0	58.0	58.4	502-542 bp	1.0	84.1
psaI-ycf4_p5	GGGCTTAGTATTTCCGGCAA	CGTTCTGATCGCCAACTCAT	50.0	50.0	58.0	58.4	511-551 bp	1.0	83.8