Hotspot

accD-psaI

LSC · 62434-63268 · 835 bp

Back to Sorbaria

Pi 0.0619

MarkerSeek score 84.50

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 835
variable_sites: 76
pi: 0.0619
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 84.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	CGGCTACATGCTTTCTTTCC	AGGGGAAGCTGTTGAGAGTT	50.0	50.0	57.2	58.6	890-896 bp	1.0	79.7
accD-psaI_p2	CGGCTACATGCTTTCTTTCC	GAGGGGAAGCTGTTGAGAGT	50.0	55.0	57.2	59.0	891-897 bp	1.0	76.0
accD-psaI_p3	CGGCTACATGCTTTCTTTCC	GGGGAAGCTGTTGAGAGTTA	50.0	50.0	57.2	56.8	889-895 bp	1.0	74.9
accD-psaI_p4	CGGCTACATGCTTTCTTTCC	AGGGGAAGCTGTTGAGAGT	50.0	52.6	57.2	57.8	890-896 bp	1.0	74.8
accD-psaI_p5	CGGCTACATGCTTTCTTTCCT	AGGGGAAGCTGTTGAGAGTT	47.6	50.0	58.6	58.6	890-896 bp	1.0	73.2