Hotspot

rbcL-accD

LSC · 60358-60990 · 633 bp

Back to Sorbaria

Pi 0.063

MarkerSeek score 87.80

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 633
variable_sites: 58
pi: 0.063
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 87.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	GCTGCTGCTTGTGAAGTATG	CCCCCGTTAAACAACATCGA	50.0	50.0	57.5	58.2	729-752 bp	1.0	84.4
rbcL-accD_p2	GCTGCTGCTTGTGAAGTATG	CGAATCGAACCGCCATTTTT	50.0	45.0	57.5	57.7	712-735 bp	1.0	84.1
rbcL-accD_p3	GCTGCTGCTTGTGAAGTATG	ATCGAATCGAACCGCCATTT	50.0	45.0	57.5	58.3	714-737 bp	1.0	83.4
rbcL-accD_p4	GCTGCTGCTTGTGAAGTATG	ATCGAACCGCCATTTTTCCA	50.0	45.0	57.5	58.5	709-732 bp	1.0	82.5
rbcL-accD_p5	GCTGCTGCTTGTGAAGTATG	TCGAACCGCCATTTTTCCAT	50.0	45.0	57.5	58.5	708-731 bp	1.0	82.5