Hotspot

trnM-CAU-atpE

LSC · 55976-56243 · 268 bp

Back to Sorbaria

Pi 0.0572

MarkerSeek score 75.40

Primer available yes

Label trnM-CAU-atpE

Feature Metadata

label_name: trnM-CAU-atpE
feature_type: igs
region: LSC
length_bp: 268
variable_sites: 23
pi: 0.0572
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnM-CAU-atpE_p1	GTTTCTATCGCCCTGCTTGA	CGTATTACCAAATCACGCGC	50.0	50.0	58.0	58.0	1181-1182 bp	0.666667	67.1
trnM-CAU-atpE_p2	GTTTCTATCGCCCTGCTTGA	GCGTATTACCAAATCACGCG	50.0	50.0	58.0	58.0	1182-1183 bp	0.666667	67.1
trnM-CAU-atpE_p3	AGTTTCTATCGCCCTGCTTG	CGTATTACCAAATCACGCGC	50.0	50.0	58.0	58.0	1182-1183 bp	0.666667	67.0
trnM-CAU-atpE_p4	AGTTTCTATCGCCCTGCTTG	GCGTATTACCAAATCACGCG	50.0	50.0	58.0	58.0	1183-1184 bp	0.666667	67.0
trnM-CAU-atpE_p5	GTTTCTATCGCCCTGCTTGA	TACGCCTTAACGACCAATGG	50.0	50.0	58.0	58.0	1125-1126 bp	0.666667	67.0