Hotspot

psbI-trnS-GCU

LSC · 8648-8801 · 154 bp

Back to Sorbaria

Pi 0.0523

MarkerSeek score 77.40

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: LSC
length_bp: 154
variable_sites: 12
pi: 0.0523
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 77.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	CGTAATCCTGGACGTGAAGA	TAAAGCGCCGGATTGCTAAT	50.0	45.0	57.1	58.0	226-246 bp	1.0	80.2
psbI-trnS-GCU_p2	CGTAATCCTGGACGTGAAGA	CAATTGGGAGGAGAGATGGC	50.0	55.0	57.1	58.0	255-275 bp	1.0	79.9
psbI-trnS-GCU_p3	CGTAATCCTGGACGTGAAGA	AATTGGGAGGAGAGATGGCT	50.0	50.0	57.1	58.1	254-274 bp	1.0	79.4
psbI-trnS-GCU_p4	CGTAATCCTGGACGTGAAGA	TTTTTCGTACCGAGGGTTCG	50.0	50.0	57.1	58.2	194-214 bp	1.0	79.4
psbI-trnS-GCU_p5	CGTAATCCTGGACGTGAAGAA	CAATTGGGAGGAGAGATGGC	47.6	55.0	57.8	58.0	255-275 bp	1.0	77.6