Hotspot

trnK-UUU-rps16

LSC · 4619-5655 · 1037 bp

Back to Sorbaria

Pi 0.049

MarkerSeek score 81.50

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 1037
variable_sites: 76
pi: 0.049
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 81.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	CCTTTCAGGATCAGTCGTGG	ATTTCAAAAAGGGCGGGAGT	55.0	45.0	58.0	58.0	1223-1229 bp	0.666667	67.0
trnK-UUU-rps16_p2	CCTTTCAGGATCAGTCGTGG	AAAGGAGACAGGCAAAAGGG	55.0	50.0	58.0	58.0	1721-1726 bp	0.666667	66.9
trnK-UUU-rps16_p3	CCTTTCAGGATCAGTCGTGG	AAAAAGGCGCTCAACCTACA	55.0	45.0	58.0	58.0	1258-1264 bp	0.666667	66.9
trnK-UUU-rps16_p4	CCTTTCAGGATCAGTCGTGG	CGACGAGAGGGAAGAGATCT	55.0	55.0	58.0	58.0	1352-1358 bp	0.666667	66.7
trnK-UUU-rps16_p5	AACGGACTCTCTAGCCTCTC	AAAGGAGACAGGCAAAAGGG	55.0	50.0	58.0	58.0	2232-2237 bp	0.666667	66.6