Hotspot

trnH-GUG-psbA

LSC · 169-670 · 502 bp

Back to Sorbaria

Pi 0.0657
MarkerSeek score 80.40
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
502
variable_sites
48
pi
0.0657
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.98
markerseek_score
80.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GATCCACTTGGCTACATCCG ATTCCAAGCTGAGCACAACA 55.0 45.0 58.1 58.0 889-1068 bp 1.0 87.4
trnH-GUG-psbA_p2 GATCCACTTGGCTACATCCG TTCCAAGCTGAGCACAACAT 55.0 45.0 58.1 58.0 888-1067 bp 1.0 87.4
trnH-GUG-psbA_p3 GATCCACTTGGCTACATCCG CTGACGGTATGCCTCTAGGA 55.0 55.0 58.1 58.0 938-1117 bp 1.0 87.3
trnH-GUG-psbA_p4 GATCCACTTGGCTACATCCG TCTGACGGTATGCCTCTAGG 55.0 55.0 58.1 58.0 939-1118 bp 1.0 87.3
trnH-GUG-psbA_p5 CAATCCACTGCCTTGATCCA TTCCAAGCTGAGCACAACAT 50.0 45.0 57.9 58.0 902-1081 bp 1.0 87.3