primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	GATCCACTTGGCTACATCCG	ATTCCAAGCTGAGCACAACA	20	20	55.0	45.0	58.1	58.0	0.0	7.4	0.143447	169	670	889-1068	1.000000	87.4
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	GATCCACTTGGCTACATCCG	TTCCAAGCTGAGCACAACAT	20	20	55.0	45.0	58.1	58.0	0.0	7.4	0.143447	169	670	888-1067	1.000000	87.4
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	GATCCACTTGGCTACATCCG	CTGACGGTATGCCTCTAGGA	20	20	55.0	55.0	58.1	58.0	0.0	0.0	0.146260	169	670	938-1117	1.000000	87.3
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	GATCCACTTGGCTACATCCG	TCTGACGGTATGCCTCTAGG	20	20	55.0	55.0	58.1	58.0	0.0	0.0	0.146260	169	670	939-1118	1.000000	87.3
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	CAATCCACTGCCTTGATCCA	TTCCAAGCTGAGCACAACAT	20	20	50.0	45.0	57.9	58.0	0.0	7.4	0.147808	169	670	902-1081	1.000000	87.3
psbA-trnK-UUU_p1	psbA-trnK-UUU	1	TCCAGTTACAAAAGCGACCC	ACTCCATCCGACTAGTTCCG	20	20	50.0	55.0	58.1	58.6	0.0	0.0	0.722548	1733	1979	349-416	1.000000	79.6
psbA-trnK-UUU_p2	psbA-trnK-UUU	2	TCCAGTTACAAAAGCGACCC	ACTAGTTCCGGGTTCGAGTC	20	20	50.0	55.0	58.1	58.8	0.0	0.0	0.940938	1733	1979	339-406	1.000000	78.0
psbA-trnK-UUU_p3	psbA-trnK-UUU	3	TCCAGTTACAAAAGCGACCC	GACTAGTTCCGGGTTCGAGT	20	20	50.0	55.0	58.1	58.8	0.0	0.0	0.940938	1733	1979	340-407	1.000000	78.0
psbA-trnK-UUU_p4	psbA-trnK-UUU	4	ACGGTTTTCAGTGCTGGTTA	ACTCCATCCGACTAGTTCCG	20	20	45.0	55.0	57.7	58.6	0.0	0.0	0.944750	1733	1979	369-436	1.000000	77.5
psbA-trnK-UUU_p5	psbA-trnK-UUU	5	TCCAGTTACAAAAGCGACCC	ATCCGACTAGTTCCGGGTTC	20	20	50.0	55.0	58.1	58.9	0.0	19.6	1.003505	1733	1979	344-411	1.000000	77.4
trnK-UUU_p1	trnK-UUU	1	CCCAAGCACACGAATTCTCT	TGGTTTTGATTGCGTTGTGC	20	20	50.0	45.0	58.2	58.4	3.5	0.0	0.621182	1980	4618	2942-2950	1.000000	82.5
trnK-UUU_p2	trnK-UUU	2	CTCCCAAGCACACGAATTCT	TGGTTTTGATTGCGTTGTGC	20	20	50.0	45.0	58.2	58.4	3.5	0.0	0.621182	1980	4618	2944-2952	1.000000	82.5
trnK-UUU_p3	trnK-UUU	3	TCCCAAGCACACGAATTCTC	TGGTTTTGATTGCGTTGTGC	20	20	50.0	45.0	58.2	58.4	3.5	0.0	0.626284	1980	4618	2943-2951	1.000000	82.5
trnK-UUU_p4	trnK-UUU	4	ACTCCCAAGCACACGAATTC	TGGTTTTGATTGCGTTGTGC	20	20	50.0	45.0	58.5	58.4	0.0	0.0	0.910937	1980	4618	2945-2953	1.000000	80.2
trnK-UUU_p5	trnK-UUU	5	GACTCCCAAGCACACGAATT	TGGTTTTGATTGCGTTGTGC	20	20	50.0	45.0	58.5	58.4	0.0	0.0	0.910937	1980	4618	2953-2954	0.666667	59.7
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	CCTTTCAGGATCAGTCGTGG	ATTTCAAAAAGGGCGGGAGT	20	20	55.0	45.0	58.0	58.0	0.0	0.0	0.025417	4619	5655	1223-1229	0.666667	67.0
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	CCTTTCAGGATCAGTCGTGG	AAAGGAGACAGGCAAAAGGG	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.016812	4619	5655	1721-1726	0.666667	66.9
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	CCTTTCAGGATCAGTCGTGG	AAAAAGGCGCTCAACCTACA	20	20	55.0	45.0	58.0	58.0	0.0	7.6	0.040076	4619	5655	1258-1264	0.666667	66.9
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	CCTTTCAGGATCAGTCGTGG	CGACGAGAGGGAAGAGATCT	20	20	55.0	55.0	58.0	58.0	0.0	0.0	0.053942	4619	5655	1352-1358	0.666667	66.7
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	AACGGACTCTCTAGCCTCTC	AAAGGAGACAGGCAAAAGGG	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.048078	4619	5655	2232-2237	0.666667	66.6
rps16_p1	rps16	1	ACTTGTGTTGGATTGGCACT	TATGTACCGACCGAACCGAT	20	20	45.0	50.0	57.9	58.3	0.0	0.0	0.392639	5656	6801	1679-1685	0.666667	63.9
rps16_p2	rps16	2	ACTTGTGTTGGATTGGCACT	CCGACCGAACCGATGATTAT	20	20	45.0	50.0	57.9	57.6	0.0	0.0	0.505646	5656	6801	1673-1679	0.666667	63.0
rps16_p3	rps16	3	AGGACTTGTGTTGGATTGGC	TATGTACCGACCGAACCGAT	20	20	50.0	50.0	58.4	58.3	0.0	0.0	0.700039	5656	6801	1682-1688	0.666667	61.4
rps16_p4	rps16	4	GACTTGTGTTGGATTGGCAC	TATGTACCGACCGAACCGAT	20	20	50.0	50.0	57.6	58.3	0.0	0.0	0.743137	5656	6801	1680-1686	0.666667	61.1
rps16_p5	rps16	5	AGGACTTGTGTTGGATTGGC	CCGACCGAACCGATGATTAT	20	20	50.0	50.0	58.4	57.6	0.0	0.0	0.813046	5656	6801	1676-1682	0.666667	60.5
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	CAGCCGATCATGTCCTTCAA	CTTACAGCAGCTTGCCAAAC	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.084514	6802	7638	1536-1577	1.000000	89.2
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	GCCGATCATGTCCTTCAAGT	CTTACAGCAGCTTGCCAAAC	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.085933	6802	7638	1534-1575	1.000000	89.2
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	CCCTTTTGCCTGTCTCCTTT	CTTACAGCAGCTTGCCAAAC	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.057170	6802	7638	2075-2096	0.666667	66.6
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	CCCTTTTGCCTGTCTCCTTT	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.0	58.1	0.0	16.9	0.077177	6802	7638	1467-1474	0.666667	66.4
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	TTCTACTCGAGCTCCATCGT	CTTACAGCAGCTTGCCAAAC	20	20	50.0	50.0	58.0	57.9	13.6	0.0	0.095912	6802	7638	1675-1696	0.666667	66.3
psbI-trnS-GCU_p1	psbI-trnS-GCU	1	CGTAATCCTGGACGTGAAGA	TAAAGCGCCGGATTGCTAAT	20	20	50.0	45.0	57.1	58.0	0.0	15.4	0.971702	8648	8801	226-246	1.000000	80.2
psbI-trnS-GCU_p2	psbI-trnS-GCU	2	CGTAATCCTGGACGTGAAGA	CAATTGGGAGGAGAGATGGC	20	20	50.0	55.0	57.1	58.0	0.0	0.0	0.955041	8648	8801	255-275	1.000000	79.9
psbI-trnS-GCU_p3	psbI-trnS-GCU	3	CGTAATCCTGGACGTGAAGA	AATTGGGAGGAGAGATGGCT	20	20	50.0	50.0	57.1	58.1	0.0	0.0	1.029055	8648	8801	254-274	1.000000	79.4
psbI-trnS-GCU_p4	psbI-trnS-GCU	4	CGTAATCCTGGACGTGAAGA	TTTTTCGTACCGAGGGTTCG	20	20	50.0	50.0	57.1	58.2	0.0	7.4	1.151451	8648	8801	194-214	1.000000	79.4
psbI-trnS-GCU_p5	psbI-trnS-GCU	5	CGTAATCCTGGACGTGAAGAA	CAATTGGGAGGAGAGATGGC	21	20	47.6	55.0	57.8	58.0	0.0	0.0	1.247891	8648	8801	255-275	1.000000	77.6
rps4-trnT-UGU_p1	rps4-trnT-UGU	1	TTTCTAAGATCGCTTCCGGC	CTCAGAGGTTAGAGCATCGC	20	20	50.0	55.0	58.1	57.9	0.0	0.0	0.196808	49343	49827	575-659	1.000000	84.6
rps4-trnT-UGU_p2	rps4-trnT-UGU	2	TTTCTAAGATCGCTTCCGGC	ATGGTCATCGGTTCGATTCC	20	20	50.0	50.0	58.1	57.8	0.0	0.0	0.304561	49343	49827	543-627	1.000000	83.8
rps4-trnT-UGU_p3	rps4-trnT-UGU	3	CTAGTTAGCCCCGGTAAAGC	CTCAGAGGTTAGAGCATCGC	20	20	55.0	55.0	57.8	57.9	0.0	0.0	0.368585	49343	49827	542-626	1.000000	83.3
rps4-trnT-UGU_p4	rps4-trnT-UGU	4	CTAGTTAGCCCCGGTAAAGC	ATGGTCATCGGTTCGATTCC	20	20	55.0	50.0	57.8	57.8	0.0	0.0	0.476338	49343	49827	510-594	1.000000	82.6
rps4-trnT-UGU_p5	rps4-trnT-UGU	5	GGGGCGAGATTGGTTTCTAA	CTCAGAGGTTAGAGCATCGC	20	20	50.0	55.0	57.6	57.9	0.0	0.0	0.546050	49343	49827	588-672	1.000000	81.8
trnV-UAC-trnM-CAU_p1	trnV-UAC-trnM-CAU	1	CCTCTGAAAAACATTGGCGC	CCCGCCGTATGAAAGCAATA	20	20	50.0	50.0	58.3	58.4	0.0	0.0	0.709622	55736	55902	272-272	1.000000	78.1
trnV-UAC-trnM-CAU_p2	trnV-UAC-trnM-CAU	2	CCTCTGAAAAACATTGGCGC	CCGCCGTATGAAAGCAATAC	20	20	50.0	50.0	58.3	57.3	0.0	0.0	0.961575	55736	55902	271-271	1.000000	76.1
trnV-UAC-trnM-CAU_p3	trnV-UAC-trnM-CAU	3	CGTGTAAACGAGGTGCTCTA	CCCGCCGTATGAAAGCAATA	20	20	50.0	50.0	57.4	58.4	0.0	0.0	1.035642	55736	55902	251-251	1.000000	75.9
trnV-UAC-trnM-CAU_p4	trnV-UAC-trnM-CAU	4	CCTCTGAAAAACATTGGCGC	TTTGAACCAATGACTCCCGC	20	20	50.0	50.0	58.3	58.8	0.0	0.0	1.054394	55736	55902	287-287	1.000000	75.2
trnV-UAC-trnM-CAU_p5	trnV-UAC-trnM-CAU	5	CTCTGAAAAACATTGGCGCG	CCCGCCGTATGAAAGCAATA	20	20	50.0	50.0	58.7	58.4	0.0	0.0	1.081020	55736	55902	271-271	1.000000	75.2
trnM-CAU-atpE_p1	trnM-CAU-atpE	1	GTTTCTATCGCCCTGCTTGA	CGTATTACCAAATCACGCGC	20	20	50.0	50.0	58.0	58.0	0.0	5.7	0.024352	55976	56243	1181-1182	0.666667	67.1
trnM-CAU-atpE_p2	trnM-CAU-atpE	2	GTTTCTATCGCCCTGCTTGA	GCGTATTACCAAATCACGCG	20	20	50.0	50.0	58.0	58.0	0.0	3.5	0.024352	55976	56243	1182-1183	0.666667	67.1
trnM-CAU-atpE_p3	trnM-CAU-atpE	3	AGTTTCTATCGCCCTGCTTG	CGTATTACCAAATCACGCGC	20	20	50.0	50.0	58.0	58.0	0.0	5.7	0.030015	55976	56243	1182-1183	0.666667	67.0
trnM-CAU-atpE_p4	trnM-CAU-atpE	4	AGTTTCTATCGCCCTGCTTG	GCGTATTACCAAATCACGCG	20	20	50.0	50.0	58.0	58.0	0.0	3.5	0.030015	55976	56243	1183-1184	0.666667	67.0
trnM-CAU-atpE_p5	trnM-CAU-atpE	5	GTTTCTATCGCCCTGCTTGA	TACGCCTTAACGACCAATGG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.037112	55976	56243	1125-1126	0.666667	67.0
rbcL-accD_p1	rbcL-accD	1	GCTGCTGCTTGTGAAGTATG	CCCCCGTTAAACAACATCGA	20	20	50.0	50.0	57.5	58.2	8.5	0.0	0.728387	60358	60990	729-752	1.000000	84.4
rbcL-accD_p2	rbcL-accD	2	GCTGCTGCTTGTGAAGTATG	CGAATCGAACCGCCATTTTT	20	20	50.0	45.0	57.5	57.7	8.5	0.0	0.795791	60358	60990	712-735	1.000000	84.1
rbcL-accD_p3	rbcL-accD	3	GCTGCTGCTTGTGAAGTATG	ATCGAATCGAACCGCCATTT	20	20	50.0	45.0	57.5	58.3	8.5	10.1	0.875652	60358	60990	714-737	1.000000	83.4
rbcL-accD_p4	rbcL-accD	4	GCTGCTGCTTGTGAAGTATG	ATCGAACCGCCATTTTTCCA	20	20	50.0	45.0	57.5	58.5	8.5	0.0	0.999079	60358	60990	709-732	1.000000	82.5
rbcL-accD_p5	rbcL-accD	5	GCTGCTGCTTGTGAAGTATG	TCGAACCGCCATTTTTCCAT	20	20	50.0	45.0	57.5	58.5	8.5	0.0	0.999079	60358	60990	708-731	1.000000	82.5
accD-psaI_p1	accD-psaI	1	CGGCTACATGCTTTCTTTCC	AGGGGAAGCTGTTGAGAGTT	20	20	50.0	50.0	57.2	58.6	0.0	0.0	1.393129	62434	63268	890-896	1.000000	79.7
accD-psaI_p2	accD-psaI	2	CGGCTACATGCTTTCTTTCC	GAGGGGAAGCTGTTGAGAGT	20	20	50.0	55.0	57.2	59.0	0.0	0.0	1.852249	62434	63268	891-897	1.000000	76.0
accD-psaI_p3	accD-psaI	3	CGGCTACATGCTTTCTTTCC	GGGGAAGCTGTTGAGAGTTA	20	20	50.0	50.0	57.2	56.8	0.0	0.0	1.984804	62434	63268	889-895	1.000000	74.9
accD-psaI_p4	accD-psaI	4	CGGCTACATGCTTTCTTTCC	AGGGGAAGCTGTTGAGAGT	20	19	50.0	52.6	57.2	57.8	0.0	0.0	2.004880	62434	63268	890-896	1.000000	74.8
accD-psaI_p5	accD-psaI	5	CGGCTACATGCTTTCTTTCCT	AGGGGAAGCTGTTGAGAGTT	21	20	47.6	50.0	58.6	58.6	0.0	0.0	2.200557	62434	63268	890-896	1.000000	73.2
psaI-ycf4_p1	psaI-ycf4	1	ATTTCCGGCAATTGCAATGG	TACGTTCTGATCGCCAACTC	20	20	45.0	50.0	58.0	57.7	30.3	8.0	0.300373	63383	63803	504-544	1.000000	84.7
psaI-ycf4_p2	psaI-ycf4	2	GGGCTTAGTATTTCCGGCAA	TACGTTCTGATCGCCAACTC	20	20	50.0	50.0	58.0	57.7	0.0	8.0	0.320371	63383	63803	513-553	1.000000	84.4
psaI-ycf4_p3	psaI-ycf4	3	GGCAATTGCAATGGCTTCTT	TACGTTCTGATCGCCAACTC	20	20	45.0	50.0	57.9	57.7	32.4	8.0	0.376907	63383	63803	498-538	1.000000	84.2
psaI-ycf4_p4	psaI-ycf4	4	ATTTCCGGCAATTGCAATGG	CGTTCTGATCGCCAACTCAT	20	20	45.0	50.0	58.0	58.4	30.3	0.0	0.381969	63383	63803	502-542	1.000000	84.1
psaI-ycf4_p5	psaI-ycf4	5	GGGCTTAGTATTTCCGGCAA	CGTTCTGATCGCCAACTCAT	20	20	50.0	50.0	58.0	58.4	0.0	0.0	0.401968	63383	63803	511-551	1.000000	83.8
ycf4-cemA_p1	ycf4-cemA	1	GCGGGTTCTCGAAAAACAAG	TCCACCAAGGCAAAAACACT	20	20	50.0	45.0	58.0	58.1	11.3	0.0	0.173112	64359	64928	1148-1153	0.666667	65.8
ycf4-cemA_p2	ycf4-cemA	2	TGCGCGTACCAATTGAAGTA	TCCACCAAGGCAAAAACACT	20	20	45.0	45.0	57.9	58.1	8.4	0.0	0.222423	64359	64928	658-663	0.666667	65.7
ycf4-cemA_p3	ycf4-cemA	3	CGTTGGGGATTTCCTGGAAA	TCCACCAAGGCAAAAACACT	20	20	50.0	45.0	58.1	58.1	23.1	0.0	0.222826	64359	64928	872-877	0.666667	65.5
ycf4-cemA_p4	ycf4-cemA	4	TTTCGTTGGGGATTTCCTGG	TCCACCAAGGCAAAAACACT	20	20	50.0	45.0	58.1	58.1	0.0	0.0	0.222826	64359	64928	875-880	0.666667	65.5
ycf4-cemA_p5	ycf4-cemA	5	TCTATGGGATCGCAGGTCTT	TCCACCAAGGCAAAAACACT	20	20	50.0	45.0	57.9	58.1	18.1	0.0	0.228802	64359	64928	985-990	0.666667	65.4
psaJ-rpl33_p1	psaJ-rpl33	1	TTTTTCCGGATGCGCTGATA	ACATCTTTACCCTTGGCCAT	20	20	45.0	45.0	58.0	56.8	4.1	6.1	1.256705	71245	71724	505-541	1.000000	80.3
psaJ-rpl33_p2	psaJ-rpl33	2	GATGCGCTGATATTCCCCTT	ACATCTTTACCCTTGGCCAT	20	20	50.0	45.0	57.8	56.8	9.7	6.1	1.416574	71245	71724	497-533	1.000000	79.1
psaJ-rpl33_p3	psaJ-rpl33	3	TTCCGGATGCGCTGATATTC	ACATCTTTACCCTTGGCCAT	20	20	50.0	45.0	58.2	56.8	4.1	6.1	1.429679	71245	71724	502-538	1.000000	79.0
psaJ-rpl33_p4	psaJ-rpl33	4	GTTCGGGTCTTTAGCAGGTT	ACATCTTTACCCTTGGCCAT	20	20	50.0	45.0	57.8	56.8	0.0	6.1	1.405879	71245	71724	546-582	1.000000	78.6
psaJ-rpl33_p5	psaJ-rpl33	5	TGGCACCGGTAGTAAGTACT	ACATCTTTACCCTTGGCCAT	20	20	50.0	45.0	57.8	56.8	6.9	6.1	1.446099	71245	71724	571-607	1.000000	78.1
rps18_p1	rps18	1	GGTTCATGGCCAAGGGTAAA	CGTTTGTGGATCACTCGGAT	20	20	50.0	50.0	58.1	58.0	11.0	0.0	0.078510	72123	72434	1151-1165	1.000000	86.1
rps18_p2	rps18	2	TTCTTCCTTGCTTCGGATCG	CGTTTGTGGATCACTCGGAT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.020290	72123	72434	1222-1222	0.666667	66.6
rps18_p3	rps18	3	TTCTTCCTTGCTTCGGATCG	GGACACGGGAAGGGAATAAC	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.044249	72123	72434	1464-1464	0.666667	66.4
rps18_p4	rps18	4	TTCTTCCTTGCTTCGGATCG	AGGACACGGGAAGGGAATAA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.049012	72123	72434	1465-1465	0.666667	66.4
rps18_p5	rps18	5	TTCTTCCTTGCTTCGGATCG	TCGTCGTTTGTGGATCACTC	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.070471	72123	72434	1226-1226	0.666667	66.2
rpl20-rps12_p1	rpl20-rps12	1	TTGTTCTACGTCTTCGAGCT	AAATGTCACGAAATCCCCCG	20	20	45.0	50.0	56.6	58.5	0.0	0.0	1.907529	73059	73847	877-902	1.000000	71.2
rpl20-rps12_p2	rpl20-rps12	2	TTGTTCTACGTCTTCGAGCT	CAGCGACGAGGAACATGTAC	20	20	45.0	55.0	56.6	58.7	0.0	0.0	2.097813	73059	73847	840-865	1.000000	69.9
rpl20-rps12_p3	rpl20-rps12	3	TTGTTCTACGTCTTCGAGCT	AGCGACGAGGAACATGTACT	20	20	45.0	50.0	56.6	58.8	0.0	0.0	2.192196	73059	73847	839-864	1.000000	69.2
rpl20-rps12_p4	rpl20-rps12	4	TTGTTCTACGTCTTCGAGCT	TCAGCGACGAGGAACATGTA	20	20	45.0	50.0	56.6	58.8	0.0	0.0	2.196537	73059	73847	841-866	1.000000	69.1
rpl20-rps12_p5	rpl20-rps12	5	TTTGTTCTACGTCTTCGAGCT	AAATGTCACGAAATCCCCCG	21	20	42.9	50.0	57.4	58.5	0.0	0.0	2.178548	73059	73847	878-903	1.000000	69.0