Hotspot

rps15-ycf1

SSC · 123685-124092 · 408 bp

Back to Sophora

Pi 0.0629

MarkerSeek score 74.30

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 408
variable_sites: 70
pi: 0.0629
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 74.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	GGTGATGAGGGCCCATATTG	TGATTTGCCCGTTCCTGAAA	55.0	45.0	58.1	58.0	1172-1200 bp	1.0	89.7
rps15-ycf1_p2	CCCAGCCATAGTAGGTGAGA	TGATTTGCCCGTTCCTGAAA	55.0	45.0	57.9	58.0	1470-1498 bp	1.0	88.5
rps15-ycf1_p3	ACAGTAGTCGTTGCCGTTTT	TGATTTGCCCGTTCCTGAAA	45.0	45.0	58.1	58.0	938-960 bp	0.888889	84.6
rps15-ycf1_p4	AACACCATGCATTGATGGGT	TGATTTGCCCGTTCCTGAAA	45.0	45.0	58.1	58.0	1196-1217 bp	0.666667	73.5
rps15-ycf1_p5	TGCATTGATGGGTGATGAGG	TGATTTGCCCGTTCCTGAAA	50.0	45.0	57.9	58.0	1189-1210 bp	0.666667	73.5