Hotspot

rpl32-trnL-UAG

SSC · 113978-114324 · 347 bp

Back to Sophora

Pi 0.0691
MarkerSeek score 74.40
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
347
variable_sites
57
pi
0.0691
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.94
markerseek_score
74.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 TCTGAATCTTCGCGCTTTCA TTCGAACCGAGATGCTCAAG 45.0 50.0 57.9 58.0 1002-1293 bp 0.888889 86.6
rpl32-trnL-UAG_p2 TCTGAATCTTCGCGCTTTCA AAATAAAATGCCATGCCGCC 45.0 45.0 57.9 58.0 1029-1320 bp 0.888889 86.1
rpl32-trnL-UAG_p3 TCTGAATCTTCGCGCTTTCA AAGCACTGCTTCCTAAGAGC 45.0 50.0 57.9 57.9 985-1276 bp 0.888889 85.8
rpl32-trnL-UAG_p4 TCTGAATCTTCGCGCTTTCA TCCTAAGAGCAGCGTGTCTA 45.0 50.0 57.9 57.9 975-1266 bp 0.888889 85.8
rpl32-trnL-UAG_p5 TCTGAATCTTCGCGCTTTCA TATCATGCCCTTTTCGGACG 45.0 50.0 57.9 58.1 1263-1430 bp 0.777778 79.5